化疗前后乳腺癌患者血清中IL-6、IL-8及TNF-α的变化及意义

Changes and significance of IL- 6,IL- 8 and TNF- α in serum of breast cancer patients before and after chemotherapy

目的:探讨化疗前后乳腺癌患者血清中IL-6、IL-8及TNF-α的变化及意义。方法:选择住院手术乳腺癌术后1个月、化疗1个疗程(21天)患者50例,化疗前、后均静脉采血,同期选取体检健康女性30例作为对照组亦静脉采血,均留取血清待测。采用ELISA酶联免疫法检测IL-6、IL-8及TNF-α的蛋白水平,采用流式细胞免疫学法检测IL-6、IL-8及TNF-α的细胞阳性百分率。结果:与对照组比较,化疗前乳腺癌组患者血清中IL-6、IL-8、TNF-α的蛋白水平及细胞阳性百分率均升高,差异有统计学意义(P<0.01);化疗后患者血清中IL-6、IL-8、TNF-α的蛋白水平及细胞阳性百分率均略升高,差异无统计学意义(P>0.05);化疗前乳腺癌组患者血清中IL-6、IL-8、TNF-α的蛋白水平及细胞阳性百分率明显高于化疗后患者,差异有统计学意义(P<0.01)。结论:化疗能够通过降低乳腺癌患者血清中IL-6、IL-8、TNF-α的水平抑制肿瘤的浸润和转移,这为乳腺癌的临床治疗提供新的靶点。

Objective： To explore the changes and significance of interleukin -6 （IL -6）, interleukin -8 （IL -8） and tumor necrosis factor - α （ TNF - α） in serum of breast cancer patients before and after chemotherapy. Methods： Fifty breast cancer patients were selected from this hospital at one month after surgery and after one course of chemotherapy, venous blood samples were obtained before and after chemotherapy; thirty healthy women after physical examination were selected during the same period, venous blood samples were ob- tained. ELISA was used to detect IL - 6, IL - 8 and TNF - α levels; flow cytometric immunological method was used to detect the percenta- ges of positive cells expressing IL - 6, IL - 8 and TNF - at. Results： Compared with control group, the levels of IL - 6, IL - 8 and TNF - at in serum and the percentages of positive ceils expressing IL - 6, IL - 8 and TNF - at in breast cancer group before chemotherapy increased significantly, there were statistically significant differences （ P 〈 0. 01 ） ; after chemotherapy, the levels of IL - 6, IL - 8 and TNF - at in ser- um and the percentages of positive cells expressing IL - 6, IL - 8 and TNF - at in breast cancer group increased slightly, there was no statis- tically significant difference （P 〉 0.05 ） ; in breast cancer group, the levels of IL - 6, IL - 8 and TNF - α in serum and the percentages of positive cells expressing IL - 6, IL - 8 and TNF - α before chemotherapy were statistically significantly higher than those after chemotherapy （P 〈 0. 01 ） . Conclusion： Chemotherapy can inhibit invasion and metastasis of breast cancer by reducing the levels of IL- 6, IL -8 and TNF - α in serum, which provides a new target for treatment of breast cancer.