茜素络合物对唐鱼耳石标记效果以及生长和存活率的影响

Effects of alizarin complexone( ALC ) on otolith marking, growth, and survival of Tanichthys albonubes

用茜素络合物（ALC）对唐鱼（Tanichthys albonubes）进行荧光标记,通过测定唐鱼耳石的标记率、死亡率、SGR和DWG等指标,讨论不同浓度ALC对唐鱼耳石标记效果和对其生长和存活的影响,探讨应用该标记技术追踪唐鱼野外种群迁移行为的可行性。结果表明：在温度28~30℃,浓度50 mg·L-1、80 mg·L-1茜素络合物溶液中浸泡24 h,唐鱼仔鱼、稚鱼死亡率为0,其中80 mg·L-1浓度处理组耳石荧光标记环检出率为100%,而在100mg·L-1浓度下,仔、稚鱼死亡率均达到80%;幼鱼在浓度为80、100 mg·L-1条件下无死亡,其中100 mg·L-1浓度处理组耳石标记环检出率为100%,而150 mg·L-1浓度下幼鱼死亡率为44%;成鱼在100、150 mg·L-1浓度下无死亡,且耳石标记率均为100%,而在200mg·L-1浓度下成鱼死亡率达到100%;此外,稚鱼在80 mg·L-1最适浓度下浸泡24 h后继续饲养90 d,标记组和对照组死亡率均为0,两组体质量、SGR和DWG差异不显著;唐鱼仔稚鱼、幼鱼和成鱼耳石标记的最适茜素络合物浓度分别为80、100和150 mg·L-1。

The effects of alizarin complexone （ALC） on otolith marking growth and survival of Tanichthys albonubes were studied, aiming to improve the applicability of this marking technique in tracing T. albonubes for a better understanding of its movement and migration pattern. Larvae, juveniles with the body length of 5.8-10.5 mm （JSs）, juveniles with the body length of 10.8- 13.2 mm （J10s）, and adults of T. albonubes were selected in the experiment, the water tempera- ture was 28-30 ℃ , and immerse time was 24 h. Larvae and J5s of T. albonubes exposed to 50 mg ·L-1 and 80 mg·L-1 of ALC had a 100% survival rate, but those exposed to 100 mg·L-1 had a mortality rate of 80%. Under the condition of 80 mg·L-1 ALC, growth rings in otoliths de- tected by fluorescence microscope （GRFM） were found in all the larvae and JSs. Under 80 mg·L-1 and 100 mg ·L-1 of ALC, all JlOs survived, but the mortality rate of JlOs exposed to 150 mg·L-1 of ALC was 44%. GRFM was found in all JlOs under 100 mg·L-1 of ALC. Under 100 mg·L-1 and 150 mg ·L-1 of ALC, all adult fish survived with GRFM, but under 200 mg·L-1 of ALC all adult fish died. Cultured for 90 days after exposure to ALC, JSs had the sim- ilar body weight, SGR, DWG, and 100% survival rate as compared to the controls （P〉0.05）. It was suggested that the suitable concentrations of ALC treating larvae, J5s, J10s, and adults of T. albonubes for GRFM were 80 mg·L-1 , 100 mg·L-1 and 150 mg·L-1 , respectively.