SIRT1基因多态性与肉牛屠宰性状相关性分析

Correlation between Polymorphisms of SIRT1 Gene and Carcass Traits in Beef Cattle

本文以牛的SIRT1基因为候选基因,采用PCR-SSCP和测序技术在利杂牛、利西杂牛、秦川牛、西杂牛和夏西杂牛5个牛群体共129头个体中检测遗传变异情况。结果表明:检测到AA、AB、AD、BD和DD共5种基因型,测序分析发现4处SNPs多态位点(Gen Bank:NM_001192980.1:g.-382A>G、-306T>C、-274G>C、-271G>C)。5个群体中不同基因型与屠宰性状的相关性分析显示,AD和BD基因型屠宰率显著高于AB、BD基因型眼肉显著高于AA、AB、AD和DD基因型,AA基因型背膘厚显著高于AA、AB、AD和DD、BD基因型净肉率显著高于AB、AD和DD基因型均达到显著水平(P<0.05)。因此,SIRT1基因单核苷酸多态性对5个牛群体的屠宰性状有一定的影响,可作为影响屠宰性状的候选基因,指导肉牛的育种工作。

In this study, we took SIRT! as a candidate gene, using PCR-SSCP and sequencing techniques to analyze genetic variation of 129 beef cattle including LZ, LXZ, QC, XZ cattle and XXZ cattle. The results showed that 5 genotypes AA, AB, AD, BD and DD and four SNPs （GenBank Access NO： NM_001192980.1： g. -382 A〉G, -306T〉 C, -274G〉C, -271G〉C） were found. Correlation analysis on different genotypes and slaughter performance indicated that in five cattle population, slaughter rate of AD and BD .genotype was significantly higher than that of AB genotype （P〈0.05）, carcass weight of BD genotype was significantly greater than that of AA type （P〈0.05）, ribeye of BD genotype was significantly greater than that of AB, AD and DD type （P〈0.05）, bone weight and meat percentage of BD genotype was significantly greater than that of AB and AD type （P〈0.05）, backfat of the AA genotype was the most thick in 5 genotypes and significantly greater than that of AB, AD, BD and DD type （P〈0.05）, and meat percentage of BD genotype was significantly higher than that of AB, AD and DD genotype （P〈0.05）. Our data suggest that the SNPs of SIRT1 gene affect on the slaughter traits of LZ, LXZ, QC, XZ and XXZ cattle, and SIRT1 could be a candidate gene to direct beef cattle breeding.