江西建昌帮炆制熟地黄中辅料作用探索(Ⅱ)

Role of Accessories in Processed Rehmanniae Radix Praeparata With Jiangxi Jianchang Medicinal Band(Ⅱ)

目的：考察江西建昌帮炆制熟地黄过程中炮制辅料的作用,为阐明炆熟地黄的炮制机制提供参考.方法：采用HPLC建立熟地黄不同炮制品的指纹图谱,流动相乙腈（A）-0.1％磷酸溶液（B）梯度洗脱（0 ～ 12 min,5％ ～10％A;12 ～40min,10％ ～ 15％ A;40 ～70 min,15％ ～ 25％A;70～ 85 min,25％～ 28％ A）,检测波长320 nm,运用《中药色谱指纹图谱相似度评价系统》2004A版分析各样品色谱图的差异性.结果：生地黄与炆熟地黄的HPLC图谱差异显著;相对于炆熟地黄,炆熟地黄（缺黄酒）、炆熟地黄（缺砂仁）、炆熟地黄（缺陈皮）、炆熟地黄（缺砂仁与陈皮）、炆熟地黄（缺砂仁、陈皮与黄酒）的相似度分别为0.844,0.741,0.727,0.717,0.717.结论：该方法操作简便、稳定性高、结果准确.在熟地黄炆制过程中,辅料会带入新化学成分并使已有成分的含量发生变化.

Objective： To investigate role of accessories in processing of Rehmanniae Radix Praeparata with Jianchang band and to offer reference for illustrating its processing mechanisms. Method： Fingerprints of Rehmanniae Radix Praeparata was determined by HPLC, mobile phase was acetonitrile （A） -0. 1% phosphoric acid solution （B） for gradient elution （0-12 min, 5%-10%A; 12-40 min, 10%-15%A; 40-70 min, 15%- 25% A; 70-85 min, 25%-28% A） and detection wavelength was 320 nm, chromatogram difference of processed products was evaluated by 2004 A version of ＇ Chinese medicine fingerprint similarity evaluation system＇. Result： There were obvious differences between Rehmanniae Radix and Rehmanniae Radix Praeparata. In contrast to Wenzhi Rehmanniae Radix Praeparata, similarity values of rice wine negative contrast, Amomi Fructus negative contrast, Citri Reticulatae Pericarpium negative contrast, Amomi Fructus and Citri Reticulatae Pericarpium negative contrast, Amomi Fructus and Citri Retieulatae Pericarpium and rice wine negative contrast were 0. 844, 0. 741, 0. 727, 0. 717, 0. 717, respectively. Conclusion： This method is simple, accurate and reproducible. It demonstrates that new components are produced or contents of known components are changed due to accessories during processing of Rehmanniae Radix Praeparata.