UFLC/Q-TOF-MS结合主成分分析法考察人参与黄连共煎前后人参皂苷类成分的变化

Evaluation of Ginsenosides Change Before and After Co-decoction of Ginseng Radix et Rhizoma and Coptidis Rhizoma by UFLC/Q-TOF-MS and Principal Component Analysis

目的:利用超快速液相-四级杆-飞行时间串联质谱(UFLC/Q-TOF-MS)结合主成分分析法考察人参-黄连共煎前后人参皂苷类成分的变化。方法:采用UPLC T3 C18色谱柱(2.1 mm×100 mm,1.8μm),流动相0.3%甲酸-0.3%甲酸乙腈梯度洗脱,负离子模式下采集质谱数据,应用Markview1.2.1等软件进行主成分分析(PCA),以黄连水煎液为空白,比较人参水煎液与人参-黄连共煎中人参皂苷类成分的变化。结果:3种溶液中发现10个差异性人参皂苷类化合物,其中齐墩果酸和2个未知化合物含量显著上升,20-葡萄糖Rf和人参皂苷Rf,Ra3,Rb1,Ra2,Rb3,Rs2含量显著下降。结论:负离子模式下方法能很好区分共煎前后人参皂苷类成分的变化,提示人参皂苷类成分水解生成的齐墩果酸型苷元可能是人参与黄连共煎后的物质基础。

Objective： To investigate change of ginsenosides before and after co-decoction of Ginseng Radix et Rhizoma and Coptidis Rhizoma by ultra-flow liquid chromatography couple with quadrupole-time-of-flight mass-spectrometry （UFLC/Q-TOF-MS） and principal component analysis （PCA）, Method; Chromatographic separation was performed on an ACQUITY UPLC T3 Cls column with a gradient elution of water-acetonitrile （containing 0.3% formic acid）. Mass spectrometer equipped with electrospray ionization source was used as detector under negative ion mode. Difference of co-decoction of Ginseng Radix et Rhizoma and Coptidis Rhizoma, Ginseng Radix et Rhizoma decoction, Coptidis Rhizoma decoction （blank） were classified by PCA with Markerview 1.2. 1 and other softwares. Result： Ten very different compound in three groups were found, contents of oleanolic acid and two unknown compounds significantly increased, while contents of 20-glucose-Rf, ginsenoside Rf, Ra3, Rbl, Ra2, Rb3, Rs2 significantly decreased. Conclusion： Samples of three groups are well classified by PCA in negative ion mode, it indicates that deglycosylation of ginsenosides may be material basis of co-decoction of Ginseng Radix et Rhizoma and Coptidis Rhizoma.