UFLC同时测定泽泻中6种三萜类成分含量

Simultaneous Determination of Six Triterpenoids in Alismatis Rhizoma by UFLC

目的：建立同时测定泽泻药材中23-乙酰泽泻醇C,泽泻醇A,24-乙酰泽泻醇A,泽泻醇G,泽泻醇B,23-乙酰泽泻醇B的UFLC含量分析方法.方法：采用Ultimate UFLC-AQ C18色谱柱（4.6 mm×100 mm,3μm）,流动相乙腈（A）-水（B）进行梯度洗脱,流速0.3 mL· min-1,二极管阵列检测器的检测波长为208,245 nm,柱温30℃.结果：23-乙酰泽泻醇C,泽泻醇A,24-乙酰泽泻醇A,泽泻醇G,泽泻醇B,23-乙酰泽泻醇B6个成分的线性范围分别为0.179 0～17.88（r=0.999 8）,0.500 0 ～100.0（r =0.999 7）,0.216 0～25.20 （r=0.999 6）,0.295 0～12.45 （r=1.000）,0.653 0～65.33（r=0.999 6）,0.393 0 ～ 78.32（r=0.999 8）mg·L-1;平均加样回收率分别为97.95％,96.70％,97.65％,96.01％,99.73％,100.3％.结论：建立的UFLC方法操作简便、快速、结果准确,适用于泽泻药材的质量控制.

Objective： To establish an ultra fast liquid chromatography （UFLC） method for simuhaneous determination of alisol C 23-acetate, alisol A, alisol A 24-acetate, alisol G, alisol B and alisol B 23- acetate in the Alismatis Rhizoma. Method： The seperation was performed on an Ultimate UFLC-AQ C18 column （2. 1 mm ×100 mm,3μ） using gradient elution with the mobile phase consisting of acetonitrile and water at a flow rate of 0.3 mL·min-1. The column temperature was maintained at 30 ℃ , and the DAD detection wavelengths were set at 208 nm and 245 nm. Result： The linear range of alisol C 23-acetate, alisol A, alisol A 24-acetate, alisol G, alisol B and alisol B 23-acetate were 0. 179 0-17. 88 （r =0. 999 8）, 0. 500 0-100. 0 （r =0. 999 7）, 0. 216 0- 25.20 （r=0.999 6）, 0.295 0-12.45 （r=1.000）, 0.653 0-65.33 （r =0.999 6）, 0.393 0-78.32 mg·L-1 （r =0. 999 8） , respectively. The average recoveries were 97.95% , 96.70% , 97.65% , 96.01% , 99.73% , 100.30%. Conclusion： The results indicated that the developed method could be considered to be a simple, rapid and accurate method for the quality control of Alismatis Rhizoma.