参苓白术散对抗生素引起肠道菌群失调小鼠的影响

Effects of Senlin Baishu San on the Dysbacteriosis Induced by Antibiotics in Mices

目的:观察参苓白术散对抗生素诱导的肠道菌群失调小鼠的影响。方法:SPF级BALB/c小鼠72只,自由饮用抗生素头孢曲松钠水溶液5 d,制备肠道菌群失调模型,将小鼠分为模型组、培菲康组(0.1 g·kg-1)和参苓白术散低、中、高剂量(0.1,0.2,0.4 g·kg-1)组,另设正常组,每组12只。连续给药7 d,无菌取粪便,应用平板稀释法培养各组肠道总厌氧菌、嗜酸乳杆菌、双歧杆菌、肠球菌和肠杆菌的变化,ELISA法检测血清Ig G、内毒素、血管活性肠肽(VIP)和P物质(SP)的含量。结果:与正常组比较,模型组益生菌(双歧杆菌、乳酸杆菌)显著减少(P<0.01),内毒素和SP均明显升高(P<0.01),血清Ig G及VIP明显降低(P<0.01);与模型组比较,参苓白术散低、中、高剂量组内毒素和SP较模型组降低(P<0.05),中、高剂量组益生菌、Ig G和VIP较模型组升高(P<0.05)。结论:参苓白术散能够有效调节抗生素引起的肠道菌群失调,对菌群失调小鼠具有较好的调节作用。

Objective： To investigate the effect of Shenlin Baishu San on the dysbacteriosis induced by antibiotics in mice. Method： The healthy SPF BALB/c mice were divided into the normal group, the model control group, the Peifeikang group （0.1 g·kg-1） and the low-, medium-, high-dose Shenlin Baishu San groups （0. 1, 0.2, 0.4 g·kg-1 ）. Dysbacteriosis models were induced by antibiotics ceftriaxone sodium solution for 5 days. After 7-day treatment, changes of intestinal flora with sterile take feces were observed using plate dilution method （each intestinal bacteria number was trained）. Serum immunoglobulin G （IgG）, endotoxin, vasoactive intestinal peptide （VIP） and substance P （SP） content were measured using enzyme linked immunosorbent assay. Result： The number of probiotics in the model group was signifigantly decreased as compared with that in the normal group （P 〈 0.01 ）. Serum levels of endotoxin and SP were significantly higher, serum IgG and VIP were significantly lower in model group than those in control group （P 〈 0.01 ）. The bacteria number, endotoxin, VIP and SP were decreased, serum IgG was increased in all dosages of Shenlin Baishu San groups, there was statistically significant difference as compared with model group （P 〈 0.05, P 〈 0.01 ）. Conclusion： Shenlin Baishu San could regulate the balance of intestinal flora effectively.