白色念珠菌对人口腔黏膜上皮角质细胞的作用研究

Study on the mechanism of Candida albicans on human buccal epithelial cells

目的研究白色念珠菌对人口腔黏膜上皮角质细胞的作用机制。方法将培养的口腔黏膜上皮角质细胞分别加入孢子型白色念珠菌(孢子组)和菌丝型白色念珠菌(菌丝组),另将单独培养的口腔黏膜上皮角质细胞作为阴性对照组。比较各组对口腔黏膜上皮角质细胞的黏附作用,并观察白色念珠菌对口腔黏膜上皮角质细胞的凋亡和增殖作用。结果菌丝组黏附指数(7.63±1.39)显著高于孢子组(3.47±1.04),差异有统计学意义(P<0.05);菌丝组凋亡率[(3.49±0.4)%]显著高于孢子组[(2.07±0.15)%]和阴性对照组[(1.98±0.07)%],差异有统计学意义(P<0.05),而孢子组和阴性对照组凋亡率相比差异无统计学意义(P>0.05)。菌丝组G0/G1期细胞比例[(38.17±7.83)%]显著低于孢子组[(49.47±11.41)%]和阴性对照组[(57.71±9.39)%],差异有统计学意义(P<0.05);S期[(8.54±4.03)%]、G2/M期[(20.18±9.59)%]细胞比例上升,且显著高于孢子组[(6.47±2.73)%、(10.71±3.19)%]和阴性对照组[(5.35±2.11)%、(12.38±4.35)%],差异有统计学意义(P<0.05);PI[(42.79±18.73)%]明显高于孢子组[(28.67±8.79)%]和阴性对照组[(26.87±7.64)%],差异有统计学意义(P<0.05),而孢子组和阴性对照组PI相比差异无统计学意义(P>0.05)。结论白色念珠菌能够导致口腔黏膜上皮角质细胞发生凋亡和增殖周期改变。

Objective To explore the mechanism of Candida albicans on human buccal epithelial cells. Methods The oral mucosa keratinocyte cultures were added to spore type of Candida albicans （spores group） and hyphae of Candida albicans （mycelium group）, oral mucosal epithelial cells cultured alone as negative control group. Compared adhesion effect of oral mucosal epithelial cells in each group, and observed the apoptosis and proliferation of Candida albicans to buccal epithelial cells. Results Adhesion index of mycelium group （7.63±1.39） was significantly higher than spore group （3.47±1.04）, with statistically significant difference （P〈 0.05）; the apoptosis rate [（3.49±0.4）%] was significantly higher than that in spores group [（2.07±0.15）%] and the control group [（1.98±0.07）%], with statistically significant dif-ference （P〈0.05）, while the spores group and the control group had no significant difference （P〉0.05）. The proportion of phase G0/G1 in mycelium group [（38.17±7.83）%] was significantly lower than that of spore group [（49.47±11.41）%] and the control group [（57.71±9.39）%], with statistically significant difference （P〈0.05）;the proportion of phase S [（8.54±4.03）%] and phase G2/M [（20.18±9.59）%] increased, significantly higher than those of the spores group [（6.47±2.73）%, （10.71±3.19）%] and the control group [（5.35±2.11）%, （12.38±4.35）%], with statistically significant difference （P〈 0.05）;PI [（42.79±18.73）%] was significantly higher than spore group [（28.67±8.79）%] and the control group [（26.87±7.64）%] , with statistically significant difference （P〈0.05）, while the spores group and the control group had no significant difference （P〉0.05）. Conclusion Candida albicans can cause oral mucosa keratinocyte apoptosis and proliferation cycle change.