DNA与CdS-NH_2纳米粒子的相互作用研究

The Study on the Interactions between DNA and Cd S-NH_2 Nanoparticles

采用荧光光谱、紫外光谱(UV-vis)、圆二色谱(CD)、透射电镜(TEM)、原子力显微镜(AFM)、琼脂糖凝胶电泳等技术研究Cd S-NH2-EcoRI复合物与DNA的相互作用.研究发现:Cd S-NH2纳米粒子与p BR322DNA结合后会延迟EcoRI的酶切反应.DNA的曲率和纳米粒子的粒径都是影响结合作用的因素,曲率较大的环状DNA比线性DNA能更好地与纳米粒子结合,小粒径的Cd S-NH2纳米粒子则更易结合到DNA上.并研究了DNA与Cd S-NH2纳米粒子之间的作用机理.

In this work,the interaction between DNA and CdS-NH2-EcoRI composites was investigated by infrared spectroscopy,UV-vis spectroscopy,fluorescence spectroscopy,CD spectroscopy,atomic force microscope,agarose gel electrophoresis,etc. It was shown that the restriction enzyme reaction of EcoRI would be retarded after CdS-NH2 nanoparticles combined with pBR322DNA. Both the curvature of DNA and the particle size of nanoparticles are the crucial factors influencing the groove binding. Circular-like DNA with larger curvature is more easily combined with nanoparticles than linear DNA. And CdS-NH2 nanoparticles with smaller size were more easily combined with DNA. The interaction mechanism of DNA and CdS-NH2 nanoparticles was also studied in this work.