摘要
为了深入了解油桐丙二酰单酰辅酶A酰基载体蛋白转酰基酶(Malonyl Co A:ACP transacylase,MCAT)在油脂合成代谢过程中的作用,利用RT-PCR技术克隆获得了油桐MCAT基因序列,且命名为Vf MCAT,并利用相关生物软件对该基因序列及其相应蛋白质进行了生物信息学分析。结果表明:Vf MCAT的CDS长为1 179 bp,编码392个氨基酸;该蛋白与蓖麻和亚麻的MCAT同源性均达85%,存在"GXSXG"和"GQGXQ"两个高度保守的motif,在系统进化关系上,其与蓖麻和亚麻的亲缘关系最为接近;分析其氨基酸序列后发现,Vf MCAT的分子量约为41.67 KDa,理论等电点p I为8.43,是一个含有一个跨膜结构域的可溶性稳定蛋白;对其二级和三级结构的预测结果表明,该蛋白主要以α-螺旋和无规则卷曲为主,且最有可能在线粒体中发挥其主要作用,文中由此推测,Vf MCAT在叶绿体中参与了油桐脂肪酸合成代谢。
In order to further know the function of Malonyl CoA：ACP transacylase in fatty acid synthesis metabolic of Verniciafordii, the full-lengths MCAT cDNA was cloned by RT-PCR technique from V. fordii, and named by VJMCAT. Bioinformatics analysis of VfMCAT gene and its deduced protein was done by using the related biological software. The results showed that the CDS length of VJMCAT was 1 179 bp, and encoded 392 amino acids. The protein had the highest similarity with Ricinus communis and Linum usitatissimum, up to 85%, and has two highly conserved motifs, “GXSXG” and “GQGXQ”. So it had the closest relationship with them in the phylogenetic tree. The results of amino acid sequence analysis showed that molecular mass was 41.67 KDa, and theoretical isoelectric point pI was 8.43. VfMCAT was a stable and soluble protein with one transmembrane domain. The secondary structure and tertiary structure of VfMCAT showed that the protein mainly existed a-helixes and random coils, and probably expressed in mitochondria. So the MCAT gene in Efordii should be involved into the process of fatty acid synthesis metabolic in chloroplast.
出处
《经济林研究》
北大核心
2014年第4期1-7,共7页
Non-wood Forest Research
基金
国家林业公益性行业科研专项重点项目"南方重要木本油料和药材模式树种长期育种技术研究"(201204403)
