丁螺环酮对大鼠局灶性脑挫裂伤损伤周围区域神经细胞凋亡的研究

The neural apoptosis effect of Buspirone on Brain Contusion Focus Perienchyma in rats

目的探讨5-HT1A受体激动剂丁螺环酮对大鼠局灶性脑挫裂伤(Controlled cortical impact injury,CCII)损伤周围区域神经凋亡的影响。方法 119只大鼠随机分为5组,A:空白对照组;B:空白对照+丁螺环酮组;C:假手术组;D:局灶性脑挫裂伤组+NS对照组;E:局灶性挫裂伤+丁螺环酮治疗组。参照改良Feeney's自由落体硬膜外撞击法制作局灶性脑挫裂伤模型,伤后1小时予以腹腔注射丁螺环酮(0.3mg/Kg)或等体积生理盐水(Normal saline,NS),在规定时间点,灌注取脑、石蜡包埋、切片,HE染色观察损伤周围区域皮质病理表现;免疫组化检测损伤灶周围皮层脑组织Bax、Bcl-2及Caspase-3蛋白的表达;TUNEL法检测损伤皮层凋亡细胞。结果 A、B、C组各指标阳性表现很弱,各指标没有明显的差异(P>0.05);D、E组各时间点各指标与A、B、C组比较有明显差异(P<0.01);D、E组之间各时间点各指标比较也有明显的统计学意义(P<0.05或P<0.01)。结论丁螺环酮对大鼠局灶性脑损伤后损伤灶周围皮层的神经细胞凋亡具有抑制作用。

Objective To explore the neural cells apoptosis effect of 5-HT1A receptor agonist Buspirone on controlled cortical impact injury （CCII） rat. Methods 119 adult male Wister rats weighing 250 ±25g were randomly divided into 5 groups. A： Normal control group, n=7; B： Normal control＋Buspirone group, n=7;C：Sham-operated group, n=35;D：CCII＋Normal Saline;E：CCII＋Buspirone. According to the time of take the brain, D or E group were divided into 6h, 12h, 24h, 72h, 168h, five subgroups, each 7 rats. 1 h after injury, Buspirone （0.3mg/kg） or equal volume NS were intraperitoneal injected. At the time point, the brain was taken and the pathological change of tissue around the injuried area was observed by H-E staining. The expression of Bax, BCL-2 and Caspase-3 protein were detected by immunohistochemical method. cortical cell apoptosis was detected by TUNEL method. Results There was no differences between A, B and C group （P〉0.05）. There was significant difference in each index respectively of D, E groups compared with those in A, B or C groups （P〈0.01）. the comparison between D and E of each index also had obvious statistical signifi-cance （P〈0.05 or P〈0.01）. Conclusion Acute treatment with the 5-HT1A receptor agonist Buspirone had an effect of inhibition on neural cells apoptosis in controlled cortical impact injury rats.