摘要
目的：建立同时测定复方吡拉西坦尼莫地平胶囊中卡托普利、阿司匹林、尼莫地平含量的HPLC法。方法：采用YMC-Pack Pro-C18色谱柱（250 mm ×4.6 mm,5μm）,以乙腈为流动相A、水（磷酸调节pH至2.5）为流动相B进行梯度洗脱,流速：1.0 ml.min-1,柱温：40℃,检测波长：215 nm（0~4.3 min）,276 nm（4.3~11.0 min）,235 nm（11.0~18.0 min）。结果：卡托普利、阿司匹林、尼莫地平分别在0.0547~1.6418μg（r =0.9999）,0.0553~1.6548μg（r =0.9999）,0.0777~2.3316μg（r=0.9997）范围内线性良好,平均加样回收率分别为100.69%（RSD=0.69%,n=6）,101.04%（RSD=1.05%,n=6）,102.56%（RSD=1.14%,n=6）。结论：该分析方法简便、快速、准确、重复性好,可用于复方吡拉西坦尼莫地平胶囊中卡托普利、阿司匹林、尼莫地平的含量测定。
Objective：To establish a RP-HPLC-UV wavelength switching method for the simultaneous determination of captopril, aspirin and nimodipine in compound piracetam and nimodipine capsules. Methods：The separation was carried out on a YMC-Pack Pro-C18 column（250 mm × 4. 6 mm,5μm） with acetonitrile-water（adjusting pH to 2. 5 with phosphpric acid）as the mobile phase with gra-dient elution. The flow rate was 1. 0 ml.min-1 . During 0-4. 3 min, the detection wavelength was 215 nm, during 4. 3-11. 0 min, the detection wavelength was 276 nm and during 11. 0-18. 0 min, the detection wavelength was 235 nm. The column temperature was 40℃. Results：The linear range of captopril, aspirin and nimodipine was 0. 054 7-1. 641 8 μg（r=0. 999 9）,0. 055 3-1. 654 8 μg（r=0. 999 9） and 0. 077 7-2. 331 6 μg（r=0. 999 7）, and the average recovery was 100. 69%（RSD=0. 69%,n=6）,101. 04%（RSD=1. 05%,n=6）and 102. 56%（RSD=1. 14%,n=6）, respectively. Conclusion： The method is simple, rapid and accurate, and can be used in the content determination of compound piracetam and nimodipine capsules.
出处
《中国药师》
CAS
2015年第1期41-43,共3页
China Pharmacist
