傅里叶近红外透射光谱法快速检测鼻炎喷剂中盐酸麻黄碱的含量

Rapid Detection of Ephedrine in Biyan Sprays with Fourier Transmittance Near-infrared Spectroscopy

目的：建立快速测定鼻炎啧剂中盐酸麻黄碱含量的分析方法。方法：采用傅里叶近红外（FF—NIR）液体透射光谱法采集样品的近红外光谱。以高效液相色谱法测得值为参考，运用偏最小二乘法，以一阶微分（ld）＋Norris平滑为预处理方法，谱段选择6136．38～5364．99cm-1和7038．90—6969．48cm-1建立定量校正模型。结果：定量模型校正集的R2为0．9926，校正集验证均方差（RMSEC）为1．20；验证集的R2为0．9935，外部验证均方差（RMSEP）为1．28，内部交叉验证彤为0．9869，内部交叉验证均方差（RMSECV）为1．60。结论：所建立的定量模型具有满意的拟合效果和预测能力，能快速检测鼻炎喷剂中盐酸麻黄碱的含量，可推广应用于生产过程中的在线分析。

Objective： To establish a rapid analysis method for determining the content of ephedrine in Biyan sprays. Methods： The FT-NIR spectra of the samples were collected by near-infrared liquid transmission spectroscopy. Using the HPLC analysis values as the ＇reference, a quantitative analysis model for ephedrine was established with partial least square （PLS）, the first derivative and Nor- ris smooth was used in the spectra pretreatment, and 6 136.38-5 364.99 cm-1 and 7 038.90-6 969.48 cm -1 were selected as the fre- quency ranges. Results： R2 and RMSEC of the calibration set was 0. 992 6 and 1.20, respectively. R2 and RMSEP of the vallidation set was 0. 993 5 and 1.28, respectively. R2 and RMSEP of the cross validation set was O. 986 9 and 1.60, respectively. Conclusion ： The method is rapid and non-desturctive, and can be applied in the rapid assessment and online examination of the quality of Biyan