UPLC法测定一捻金中人参皂苷Rg_1、人参皂苷Re、人参皂苷Rb_1的含量

Determination of Ginsenoside Rg_1,Ginsenoside Re and Ginsenoside Rb_1 in Yi'nianjin by UPLC

目的：建立超高效液相色谱法测定一捻金中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1含量的方法。方法：色谱柱：ZORBAX Eclipse Plus C18柱（2．1mm×50mm，1．8μm）；流速：0．21ml·min-1；柱温：30℃；流动相：乙腈-水梯度洗脱；检测波长：203nm；进样量：1μl。结果：人参皂苷Rg1在浓度为0.0203～0．3039mg·ml-1时线性关系良好（r=0．9996），平均回收率为99．05％，RSD为1．3％（n=6）；人参皂苷Re在浓度为0．0202～0．3027mg·ml。时线性关系良好（r=0．9998），平均回收率为101．31％，RSD为1．1％（n=6）；人参皂苷Rbl在浓度为0．0203—0．3051mg·ml-1时线性关系良好，r=0．9998，平均回收率为100．71％，RSD为0．9％（n=6）。结论：该方法简单、准确，可同时测定3种人参皂苷的含量，可作为一捻金的质量控制。

Objective ： To establish a UPLC method for the determination of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in Yi＇ nianjin. Methods： The column was ZORBAX Eclipse Plus C18 （2.1 mm × 50 mm, 1.8 μm）, the flow rate was 0.21 ml . min-1, the column temperature was 30℃, the mobile phase consisted of acetonitrile-water with gradient elution, the detection wave- length was 203nm, and the sample size was 1μl. Results： The linear range of ginsenoside Rgl was 0. 020 3-0.303 9 mg . ml-1 （r = 0. 999 6） , and the average recorery was 99.05% （RSD = 1.3% , n = 6）. The linear range of ginsenoside Re was 0. 020 2-0. 302 7 mg . ml - 1 （ r = 0.999 8）, and the average recovery was 101.31% （ RSD = 1.1%, n = 6）. The linear range of ginsenoside RbI was 0. 020 3-0. 305 1 mg . ml - 1 （ r = 0.999 8 ）, and the average recovery of 100.71% （ RSD = 0.9%, n = 6）. Conclusion ： The method is simple and accurate, and can determine the three components simultaneously. The method can be used in the quality control of Yi＇