HPLC同时测定竹节参中竹节参皂苷Ⅴ和Ⅳa含量

Determination of Chikusetsusaponin Ⅴ and Ⅳ a in Panax Japonicus by RP-HPLC

目的：建立HPLC同时测定竹节参中竹节参皂苷Ⅴ和Ⅳa的含量测定方法,并对不同产地的竹节参药材进行含量测定。方法：采用Hibar C18色谱柱,以乙腈-0.1%磷酸溶液为流动相,梯度洗脱,柱温30℃,检测波长203 nm。结果：竹节参皂苷Ⅴ在0.36--7.20μg（r〉0.999）范围内线性关系良好,平均加样回收率为96.0%,（RSD=0.73%,n=6）;竹节参皂苷Ⅳa在0.57~11.40μg（r〉0.999）范围内线性关系良好,平均加样回收率为104.2%（RSD=0.86%,n=6）。结论：该方法结果准确、重复性好,可用于竹节参药材的质量控制。

Objective ： To develop an RP-HPLC method for simultaneous determination of ehikusetsusaponin V and IV a in Panax japonieus of 7 different origins. Methods ： Chromatographic separation was performed on a Hibar C18 column, using a step gradient program with the mobile phase of aeetonitrile-0.1% phosphoric acid water solution. The temperature of column was 30 ℃ and the detective wavelength was 203 nm. Results ： Chikusetsusaponin V and IV a showed good linear relationships in the ranges of 0.36-7.20 μ g and 0.57-11.40 μg （r〉0.999）, respectively. The average recoveries were 96.0% （ RSD=0.73%, n=6 ） and 104.2% （ RSD=0.86%, n=6 ）, respectively. Conclusions ： The HPLC method is accurate, simple and reproduetive. It is helpful to control the quality of Panaxjaponieus.