黄连肉桂药对配伍研究

Study on compatibility of Chinese Goldthread and Cinnamon

目的:黄连和肉桂是古方交泰丸治疗心肾不交型失眠的主要组成药物,确定黄连、肉桂药对配伍的单煎合并或合煎的提取方法,以及含量测定的方法。方法:采用水煎煮法考察挥发油的最佳提取工艺,通过紫外可见分光光度法确定黄连、肉桂配伍的总生物碱含量测定的方法;通过高效液相色谱法确定黄连、肉桂配伍的有效成分含量测定的方法。结果:黄连肉桂两味药配伍以后的分煎合并溶液中总生物碱含量比黄连肉桂两味药配伍以后的合煎溶液中总生物碱含量要高;黄连肉桂两味药配伍以后的分煎合并溶液中的有效成分(包括盐酸小檗碱、盐酸药根碱、盐酸巴马汀、桂皮醛、桂皮酸)比黄连肉桂两味药配伍以后的合煎溶液中的有效成分(包括盐酸小檗碱、盐酸药根碱、盐酸巴马汀、桂皮醛、桂皮酸)要高。结论:水煎煮法是挥发油的最佳提取方法。

Objective ： Chinese Goldthread and cinnamon are the main components of ancient Jiaotai pills for heart and kidney disharmony insomnia. To determine the best compatibility ratio. Method： By making use of water extracting method, we inspected the best volatile oil extraction technology. By TLC launched agents and UV-visible spectrophotometry chromatography, we determined the conditions of groping Chinese Goldthread and cinnamon. Results： Appropriate agent can start cinnamic aldehyde, cinnamic acid, berberine and jatrorrhizine. Palmatine hydrochloride has good separation and identification of effects and suitable chromatographic conditions can be more ideal. Conclusion ： Making volatile oil by water extracting is the best method.