自制新鲜全血对Beckman HMX和Abx Pentra 60的校准应用评价

Calibration application evaluation of self-made fresh blood on Beckman HMX and Abx Pentra 60

目的使实验室内不同血细胞仪的检测结果均准确可靠且具有可比性。方法将Beckman Coulter LH750校准后作为参考仪器,抽取患者新鲜全血混匀后分为3等份,第1份在Beckman Coulter LH750仪器上确定靶值,第2份用来对Beckman HMX和Abx Pentra 60进行校准,第3份作为校准后的验证。校准完成后对患者新鲜全血进行检测,分别进行准确性评价。结果经校准后,各台仪器的RBC、HGB、WBC、HCT、MCV、PLT检测结果均有较大程度的改善,且结果间的误差均小于CLIA′88要求的1/3。结论用新鲜全血对血细胞分析仪进行校准的方法是切实可行的。

Objective To make the detection results of the different automated hematology analyzers in laboratory to be reliable and comparable. Methods With the Beckman Coulter LH750 after calibration as the reference instrument,the fresh whole blood was extracted from the patient and divided into 3 equal parts after mixing evenly. The first part was determined the target value in the LH750 instrument; the second part was used to calibrate the HMX and ABX PENTRA 60 instruments and the third part served as a calibration validation. After calibration,the fresh whole blood of patient was performed the detection for conducting the accuracy evaluation. Results After calibration,the detection results of RBC, HGB,WBC, HCT,MCV and PLT by each instrument were largely improved and the error of the results was less than 1/3 of CLIA＇88 requirements. Conclusion Adopting fresh whole blood for calibrating the hematology analvzer is feasible.