呼吸道感染患儿肺炎支原体和沙眼衣原体检测结果分析

Analysis on detection results of Mycoplasma pneumoniae and Chlamydia trachomatis in children with respiratory tract infection

目的了解呼吸道感染患儿肺炎支原体(MP)、沙眼衣原体(CT)的检出情况。方法应用荧光定量聚合酶链反应法对1 393例呼吸道感染患儿同时进行MP、CT检测。结果 MP、CT总检出率为30.4%,其中MP为21.8%,CT为9.3%,MP合并CT为0.65%。在季节分布上,夏季MP和CT检出率最高。在年龄分布上,大于或等于3岁组的患儿MP的检出率要明显高于其他年龄组,差异有统计学意义(P<0.05);小于1个月组患儿CT检出率要高于其他组,差异有统计学意义(P<0.05)。结论夏季易检出MP和CT;3岁以上患儿较其他年龄段患儿易检出MP;新生儿较其他年龄段患儿易检出CT。荧光定量聚合酶链反应法诊断支原体、衣原体感染快速、敏感,特异性高。

Objective To understand the detection situation of Mycoplasma pneumoniae （MP） and Chtarnydia trachomatis （CT） in children with respiratory tract infection. Methods MP and CT were simultaneously measured by fluorogenic quantitative PCR in 1393 children patients with respiratory tract infection. Results The total detection rates of MP and CT were 30.4% ,in which MP was 21.8% ,CT was 9.3% and MP complicating CT was 0.65%. In the season distribution,the detection rates of MP and CT were highest in summer. In the age distribution,the detection rate of MP in the over 3-year-old group was higher than that in the other age groups,the differences had statistical significance（P〈0.05）. The detection rate of CT in the less than 1-month-old group was higher than that in other age groups with statistical difference（P〈0.05）. Conclusion MP and CT are easy to be detected out in summer;MP is easy to be detected out in children over 3 years old than other age groups;MP is easy to be detected out in neonates than other age groups. Fluorogenic quantitative PCR is rapid, sensitive and highly specific for the diagnosis of MP and CT infections.