样本收集和保存对血液透析患者iPTH检测的影响

Influence of sample collection and storage on detection of iPTH in patients with hemodialysis

目的评估样本收集和保存对全段甲状旁腺素(iPTH)检测结果的影响。方法 10份血液透析患者血液样本同时注入4种采血管:促凝管、空白管、EDTA管及枸橼酸钠管,采用罗氏cobase411免疫分析仪分别检测在采血后4h内,以及4℃保存12、24、48h的血清iPTH水平。结果采血4h内促凝管血清iPTH快速发生降解,而空白管中iPTH水平最高,且明显高于12、24、48h。而在EDTA管和枸橼酸钠管,iPTH水平在48h才会开始降解。结论首次揭示了促凝剂对血液透析患者血清iPTH有降解或抑制作用,进一步证实EDTA管是iPTH最佳的采血管。

Objective To evaluate the influence of sample collection and storage on the detection results of intact parathyroid hormone（iPTH）. Methods 10 blood samples from the hemodialysis patients were simultaneously injected into four types of blood collection tube：pro-coagulation tube, blank tube, EDTA tube and sodium citrate tube. The iPTH levels in the fresh samples within 4 h after blood collection and serum/plasma preserving for 12,24,48 h under 4OC were detected by the Roche cobase411 immunoassay instrument. Results Serum iPTH in the pro-coagulation tube within 4 h after blood collection was rapidly degraded,while the iPTH level in the blank tube was highest, which was significantly higher than that the serum/plasma preserved for 12,24,48 h. The iPTH level in the EDTA tube and the sodium citrate tube began to degrade only at 48 h. Conclusion It is the first time to reveal that the coagulant has the degrading or inhibiting effect on serum iPTH, which verifies that the EDTA tube is the optimal blood collection tube.