几种银杏类制剂中银杏酸的高效液相色谱法测定

High performance liquid chromatography detection of ginkgolic acids in several kinds of ginkgo preparations

采用正己烷提取,薄层色谱纯化,高效液相色谱法(HPLC)检测并考察几种银杏类制剂总银杏酸的含量。色谱条件为Hypersil ODS C-18(150 mm×4.6 mm,5μm);流动相组成为甲醇-3%HAc溶液(92:8,V/V),紫外检测波长306 nm,流速1.0 ml/min,柱温40℃,进样量:20μl,外标法计算含量。结果表明:总银杏酸线性回归方程为:Y=4998X-100492,r2=0.9986,线性关系良好。标准品和样品的相对标准偏差RSD均为0.38%,表明仪器精密度良好。7种银杏产品中,采摘的新鲜银杏叶中总银杏酸的含量接近于1%,其余6种市场上的银杏制剂总银杏酸含量均较大程度超过10 ppm。其中,这一研究结果为正确使用银杏叶产品提供了参考。

A detection method of ginkgolic acids in ginkgo preparations by high performance liquid chromatography （HPLC） was established with extraction of n-hexane and purification of thin-layer chromatography. The chromatographic condition was Hypersil ODS C-18 （150 mm × 4.6 mm, 5 μm）. The composition of mobile phase was methanol-3% HAc solution （92：8, V/V）. Ultraviolet detection wavelength was 306 nm, flow velocity was 1.0 ml/min, column temperature was 40℃ , and sample amount was 20 μl. The content was calculated by external standard method. The results showed that the linear regression equation of ginkgolic acids was Y=4998X-100492, r2=0.9986, and the linear relation was good. The relative standard deviations RSD of standard and extraction samples were all 0.38%, which showed the instrument had good precision. In 7 ginkgo samples, the content of ginkgolic acids in fresh ginkgo leaf was 1%, and that of the other 6 ginkgo drugs were all above 10 ppm. This study provided references for correct, use of ginkgo preparations.