酪氨酸蛋白激酶抑制剂联合放疗抑制乳腺癌细胞增殖以及荷瘤裸鼠肿瘤生长

The tyrosine kinase inhibitors in combination with radiotherapy enhance the inhibition of breast cancer cells and breast cancer bearing nude mice

目的研究酪氨酸蛋白激酶抑制剂(TKI)联合放疗对乳腺癌细胞增殖及肿瘤生长的影响。方法利用噻唑蓝(MTT)实验检测不同浓度的TKI对乳腺癌细胞MCF-7的抑制作用。将乳腺癌细胞分为生理盐水组,TKI干预组(TKI处理后无X射线照射),X射线干预组(X射线照射,无TKI处理)和TKI联合放疗干预组。利用克隆形成实验比较各组细胞存活率。构建乳腺癌裸鼠异位瘤模型,考察不同干预组对肿瘤生长的抑制能力。结果克隆形成实验显示,单独利用X射线照射或者单独利用任何浓度的TKI抑制剂处理乳腺癌细胞,细胞的存活率均较处理前出现降低;但TKI联合放疗乳腺癌细胞存活率较前面两组(TKI干预组,X射线干预组)差异有统计学意义(P<0.05)。与TKI预处理组或X射线干预组比较,TKI联合放疗干预组能够明显抑制荷瘤裸鼠肿瘤体积的增长,差异有统计学意义(P<0.05)。结论 TKI抑制剂联合放疗可有效抑制乳腺癌细胞的生长。

Objective In order to research the effect of tyrosine kinase inhibitor in combination with radiotherapy on the inhibition of breast cancer cells and breast cancer bearing nude mice .Methods Methyl thiazolyl tetrazolium （MTT） assay was used to evaluate the inhibition of different concentrations of TKI on breast cancer cells ,the breast cancer cells was divided into three groups ：the TKI treatment group ,the cells in the control group （no the TKI processing） and the control group （non-TKI and X-ray irradiation group） .The sensitivity of the cells in each group to X-ray was compared by colony formation assay .MCF7 cells were xenografted in athymic nude mice to establish the animal model ,which was used to evaluate the effect of anticancer .Results Colony form-ing test showed that the separated use of any concentrations of the TKI inhibitor could inhibit the breast cells ,and the cell viability was significantly reduced;TKI combined with X-ray irradiation could significantly increase the sensitivity of breast cancer cells to radiation ,and the difference was statistically significant（P〈0 .05） .Compared to TKI inhibitor or X-ray irradiation alone ,the combination of TKI inhibitor with X-ray irradiation could inhibit the growth of tumor effectively .Conclusion The TKI inhibitor in com-bination with radiotherapy can effectively inhibit the growth of breast cancer cells ,which provides a new theoretical basis for the implementation of the clinical breast cancer radio sensitization .