核磁共振法测定甲哌鎓原药的含量

Determination of the Content of Mepiquat Chloride by Quantitative Nuclear Magnetic Resonance Spectrometry

采用定量核磁共振法建立了甲哌鎓原药的定量方法。以重水为溶剂,仲丁基脲为内标物,以化学位移分别在δ3.12的甲哌鎓甲基和δ1.10的仲丁基脲甲基的1H-NMR信号作为定量峰。以甲哌鎓与仲丁基脲定量峰的峰面积比(AS/AI)对甲哌鎓与仲丁基脲的质量比(WS/WI)进行线性回归,线性相关系数为0.999 9;重复测定同一批次原药含量,变异系数为0.28%。采用核磁共振波谱法对甲哌鎓进行定量分析,操作简便,测定速度快,准确度高。

A quantitative analytical method for Mepiquat chloride by nuclear magnetic resonance spectrometry was developed.The heavy water was used as solvent and 1-（sec-butyl） urea was used as internal standard.The methyl peak at 3.12 of Mepiquat chloride and the methyl peak at 1.10 of 1-（sec-butyl） urea were used for quantification.Linear regression of quantitative peak area ratio （AS/AI） versus mass ratio （WS/WI） of Mepiquat chloride to 1-（sec-butyl） urea yielded a correlation coefficient of 0.999 9.The variation coefficient within the same batch sample was 0.28％.This method could be used to determining the content of Mepiquat chloride quickly and accurately.