涤毒灌肠方对5/6肾切除大鼠血与尿硫酸吲哚酚含量的影响

Effect of Didu decoction enema on indoxyl sulfate level in serum and urine in 5/6 nephrectomized rats

目的探讨涤毒灌肠方对5/6肾切除大鼠血、尿硫酸吲哚酚含量的影响。方法 SD大鼠80只,随机抽取8只作为空白对照组,其余72只行5/6肾切除术复制慢性肾衰模型,将造模成功的60只大鼠随机分为模型对照组、药用炭治疗组、涤毒灌肠方高、中、低剂量组,用药周期为56 d。测量大鼠血清肌酐、尿素氮,高效液相色谱-质谱分析法测定血、尿硫酸吲哚酚的含量。结果灌肠方各剂量组和药用炭组血硫酸吲哚酚含量明显下降(P<0.05),且高、中剂量组比药用炭组下降更为明显(P<0.05);灌肠方高、中剂量组、药用炭组尿硫酸吲哚酚含量明显下降(P<0.05),且高剂量组比药用炭组更为明显(P<0.05)。结论涤毒灌肠方降低5/6肾切除大鼠血、尿中硫酸吲哚酚含量可能是其干预肾衰竭的作用机制之一。

Objective To study the effect of Didu decoction enema therapy on indoxyl sulfate content in serum and urine in 5 / 6 nephrectomy rats. Methods Eight SD rats were randomly selected as the control group and 72 SD rats were carried on 5 / 6 nephrectomy. Sixty rats,which were successfully modeled,were divided into the pathological control group,the medicinal charcoal tablets treatment group and three Didu enema treatment groups with high,middle and low doses. After 56 days treatment,the creatinine and urea nitrogen content in serum were measured,and the content of indoxyl sulfate in serum and urine was determined by HPLC-MS. Results The contents of creatinine and urea nitrogen in serum and the level in serum indoxyl sulfate were significantly decreased in the Didu decoction enema therapy groups and the medicinal charcoal tablets treatment group（ P〈0. 05）. Moreover,the high and middle dose groups were better than the medicinal charcoal tablets treatment group（ P〈0.05）. The content of urinary indoxyl sulfate decreased significantly in high and middle dose groups and the medicinal charcoal tablets treatment group（ P0.05）. Moreover,the high dose group was better than the medicinal charcoal tablets treatment group（ P0.05）. Conclusion Didu decoction enema may play a protective effect on renal failure by decreasing the content of indoxyl sulfate content in serum and urine.