猪心包塑形三瓣叶管道的制备及其体外评价

Porcine pericardium three valve conduit preparation and in vitro evaluation

目的制备出猪心包塑形三瓣叶管道，在体外条件下检测其抗反流性、力学强度、抗酶降解能力、血液相容性和免疫原性，并与牛颈静脉三瓣管道相比较，以探讨其临床应用前景。方法以心包制备三瓣叶管道共计12根，并取与猪心包三瓣叶管道直径大致相同的带瓣牛颈静脉8根，通过 HE染色、Masson 三色染色和 Elastica-vall-Gienson 染色粗略了解管道的免疫原性；以蠕动泵模拟心脏搏动以评价管道的抗反流特性；计算最大拉伸强度和断裂伸长率来评价力学性能；以胶原蛋白酶降解管道来比较抗酶降解的能力；以体外溶血率比较管道的血液相容性。结果①牛颈静脉管道组（A 组）的溶解百分比为30．12％±2．37％，猪心包管道组（B 组）的溶解百分比为36．27％±4．62％；②蠕动泵转数为30 r／min 时，A 组反流比率为24．17％±10．53％，B 组为18．91％±13．48％，50 r／min 时，A 组反流比率为47．6％±9．8％，B 组为44．9％±9．64％，70 r／min 时，A 组反流率为57．2％±10．7％，B 组为53．3％±19.4％；③横轴血管片最大拉伸强度分别为 A 组（2．82±1．2）N ／mm2、B 组为（11．84±4．69）N ／mm2，断裂伸长率分别为 A 组78．43％±19．24％、B 组49．26％±11．27％，纵轴血管片最大拉伸强度 A 组（1.92±1．25）N ／mm2、B 组（13．29±5．08）N ／mm2，纵轴断裂延伸率分别为 A 组77．07％±18．74％、B组49.72％±15．85％；④A 组溶血率为0．14％±0．465％，B 组溶血率0．0975％±0．445％。结论自制的猪心包带瓣管道，大体和微观形态无明显改变，在抗反流性能上与牛颈静脉无明显差异，机械性能明显优于牛颈静脉，短期抗酶降解能力较牛颈静脉略有不足，免疫原性上优于牛颈静脉管道，具备良好的血液相容性。

Objective Prepared porcine pericardium three valve conduit,and in vitro conditions,de-tected the anti-reflux,mechanical strength,resistance to enzyme degradation ability,blood compatibility and immunogenicity,and compared with bovine jugular vein,in order to discuss the clinical application.Methods Porcine pericardium sewn three valve conduit a total of 12 pipelines.Take the same conduit diameter valve bo-vine jugular vein a total of 8,doing the HE,Masson and Elastica-vall-Gienson stained light microscopy test to observe the changes in morphology and sketchy understanding conduit immunogenicity,use peristaltic pump simulating heart throb to evaluate the anti-reflux.Doing a Villa extended experiment to draw the stress-strain curves,then calculating the slope k,evaluate the mechanical properties,degrading them in the circumstance of collagen type I to understand the capacity of resistance to enzymatic degradation,detecting the rate of haematol-ysis to see whether they fit the principal of hemolytic compatibility.Results （1）Dissolved percentagethe for group A 30.12% ±2.37%,group B 36.27% ±4.62%;（2）Peristaltic pump for 30 r/min,A group of reflux ratio for 24.17% ±10.53%,as group B 18.91% ±13.48%,;50 r/min,A group of reflux ratio for 47.6%±9.8%,group B 44.9% ±9.64%;70 r/min,A group for 57.2% ±10.7%,group B 53.3% ±19.4%;（3）The horizontal axis of the maximum tensile strength of vascular piece for A group（2.82 ±1.2）N /mm2 , group B（11.84 ±4.69）N /mm2 ,elongation ratio at break for A group 78.43% ±19.24%,group B 49.26%±11.27%,the vertical axis of maximum tensile strength of vascular piece for group A（1.92 ±1.25）N /mm2 , group B（13.29 ±5.08）N /mm2 ,elongation ratio for group A 77.07% ±18.74%,group B 49.72% ±15.85%;（4）Hemolysis rate for group A 0.14% ±0.465%,group B 0.0975% ±0.445%. Conclusion Porcine pericardium three valve conduit had no significant difference in morphology when compared with the bo-vine jugular vein pipeline.In resistance to reflux performance,porcine pericardium three valve conduits and bo-vine jugular veins are no obvious difference,mechanical performance and the immunogenicity better than bovine jugular vein,short-term resistance to enzyme degradation ability is insufficient slightly.They all have a good blood compatibility.