摘要
An improved synchronous fluorimetric method for the determination of dissolved organic matter in cave drip water, by adding ascorbic acid, is described. The method is based on the redox reaction between ascorbic acid and the electron-withdrawing constituents in dissolved organic matter. The results show that adding ascorbic acid can quench the minor peaks, at 200-300 nm, but does not affect the intensity of the main peaks at 300-500nm. In addition, adding ascorbic acid can maintain relatively high and constant fuorescence intensity over a wide pH range (9-4).
An improved synchronous fluorimetric method for the determination of dissolved organic matter in cave drip water, by adding ascorbic acid, is described. The method is based on the redox reaction between ascorbic acid and the electron-withdrawing constituents in dissolved organic matter. The results show that adding ascorbic acid can quench the minor peaks, at 200-300 nm, but does not affect the intensity of the main peaks at 300-500nm. In addition, adding ascorbic acid can maintain relatively high and constant fuorescence intensity over a wide pH range (9-4).
基金
Acknowledgements This work was supported by the National Natural Science Foundation of China (Grant Nos. 41072262, 41371216, and 41302145), China Postdoctoral Science Foundation (Grant No. 2013M531757). We are grateful to Prof. Philip A. Meyers and James R. Maxwell for language editing that notably improved the manuscript.