家蚕黑胸败血芽孢杆菌基因组测序及结构分析和功能注释

Genome Sequencing,Structural Analysis and Functional Annotations of Bacillus bombysepticus from the Silkworm,Bombyx mori

黑胸败血芽孢杆菌(Bacillus bombyseptieus)是家蚕细菌性败血病的常见病原之一。在获得黑胸败血芽孢杆菌基因组完成图的基础上,对该菌株的基因组测序、基因组序列结构特征以及基因功能注释等信息进行分析。该菌株的基因组测序数据量达到2.1 Gb,基因组覆盖度超过360倍,基因组组装大小为5.87 Mb,包含2个复制子——1个核基因组(5 295.783 kb)和1个质粒基因组(577.809 kb);基因组重复序列比率为1.179 2%,包含136个非编码RNA和5 298个编码基因。测序得到的基因组数据通过KEGG和COG数据库进行功能注释,主要与物质转运、氨基酸代谢、碳水化合物代谢等相关。基因组共线性分析表明黑胸败血芽孢杆菌与芽孢杆菌属细菌的亲缘关系较近。该研究结果将为鉴定黑胸败血芽孢杆菌的毒力因子以及研究其致病机制和与宿主的相互作用提供重要数据支撑。

Bacillus bombyseptieus is one of the common bacterial pathogens causing bacterial black thorax septicemia in silkworm（ Bombyx mori）. In this paper,sequence features as well as structural and functional annotations of B. bombyseptieus genome were focused based on sequencing and assembly of B. bombyseptieus genomic sequences. We generated 2. 1 Gb of raw data from sequencing,with a coverage of more than 360-fold to the genome,and assembled them into a 5. 87 Mb genome of B. bombyseptieus,including two replicons,one of nuclear genome（ 5 295. 783 kb） and one of plasmid genome（ 577. 809 kb）. Percentage of repeat sequences accounts for 1. 179 2% in the nuclear genome,which contains 136 non-coding RNAs and 5 298 predicted protein-coding genes. The functional annotation of B. bombyseptieus genome using KEGG and COG databases showed that most genes are involved in transportation,amino acid metabolism and carbohydrate metabolism. Genomic syntenic analysis indicated that B. bombyseptieus is closely related to bacteria of Bacillus genus. These results will help find the key virulence factor of B. bombyseptieus and facilitate the understanding of its infection mechanism and interaction with host.