2种家蚕病原性微孢子虫的蛋白质组差异研究(英文)

Differential Proteomic Analysis of Two Pathogenic Microsporidia,Nosema bombycis and Endoreticulatus sp. Zhenjiang Isolated from Silkworm,Bombyx mori

微孢子虫是一类专性细胞内寄生的单细胞真核生物,几乎可以感染从无脊椎动物到脊椎动物的所有动物。对分别属于微粒子虫属(Nosema)和内网孢虫属(Endoreticulatus)的2种家蚕病原性微孢子虫的蛋白质组差异进行研究,探讨二者对家蚕侵染力差异产生的原因。采用双向电泳技术对提取的2种微孢子虫的总蛋白质进行分离,经Image Master 2D软件分析总蛋白质的双向电泳图谱发现142个差异蛋白点,其中67个蛋白点在家蚕微孢子虫中高量表达,75个蛋白点在内网孢虫属微孢子虫镇江株中高量表达。对这些差异蛋白点进行MALDI-TOF/PRO质谱鉴定,并用MASCOT软件在NCBI公共数据库中寻找匹配蛋白,去掉重复数据后最终分别在家蚕微孢子虫和内网孢虫属微孢子虫镇江株中鉴定了43个、68个有功能注释的蛋白质,其功能涉及侵染、能量代谢、孢壁组成和遗传信息加工等方面。值得注意的是鉴定到的极管蛋白3(PTP3)仅在家蚕微孢子虫高量表达。

Microsporidia are unicellular eukaryotes and obligate intracellular parasites. Their host range is extremely wide,extending from invertebrates to vertebrates. Through comparing proteomic difference of two pathogenic microsporidia which are both isolated from silkworm（ Bombyx mori） and belong to the genera Nosema and Endoreticulatus respectively,we explored the reason of pathogenicity difference between these two microsporidia to silkworm. Total proteins of the two microsporidia were separated by two-dimensional electrophoresis（ 2-DE） of which image was analyzed by Image Master 2D software. Among the 142 differential protein spots,67 protein points were highly expressed in N.bombycis,and 75 protein points were highly expressed in Endoreticulatus sp. Zhenjiang. All the 142 protein spots were sent to MALDI-TOF/ PRO for identification. After excluding the repeated matches,43 proteins of N. bombycis and 68 proteins of Endoreticulatus sp. Zhenjiang were finally identified. They were found to be involved in host cell invasion,energy transformation,spore wall composition,genetic information processing and so on. More interestingly,polar tube protein 3（ PTP3） was only highly expressed in N. bombycis.