丽蚌草组织培养及植株再生

Tissue culture and plant regeneration of Arrhenatherum elatius

以丽蚌草(Arrhenatherum elatius var.tuberosum‘Variegatum’)茎叶连接的组织为外植体,探讨不同培养基对丽蚌草愈伤组织的诱导、生芽诱导及生根的影响。结果表明:当培养基为B5+2,4-D 4 mg/L+CH 0.3 g/L时,丽蚌草叶片诱导愈伤组织效果最好;当培养基为MS+6-BA 5 mg/L+NAA 2 mg/L时,丽蚌草愈伤组织生芽效果较好;当培养基为1/2MS+6-BA 0.1 mg/L+NAA 1.0 mg/L+0.1%活性炭时,生根效果最好。

The influences of different culture medium on the induced callus, induced buds and roots of A rrhenatherum elatius with the leaves as explants were explored. The results show that the optimum induced callus medium was B5＋2,4-D 4 mg/L＋CH 0.3 g/L; the optimum induced buds medium was MS＋6-BA 5 mg/L＋NAA 2 mg/L and the optimum induced roots medium was 1/2MS＋6-BA 0.1 mg/L＋NAA 1.0 mg/L＋0.1% acticarbon.