正交试验优化红豆杉硒多糖的提取

Extraction of Se-polysaccharides from Taxus chinensis by orthogonal test

以恩施高硒地区的珍贵药用植物红豆杉为研究对象,以料液比、水浴浸提时间以及微波处理时间为试验因素设计正交试验,优化了红豆杉硒多糖的提取分离纯化工艺。通过硫酸苯酚法测定红豆杉中硒多糖的含量,采用原子荧光法测定硒多糖中硒的含量,并以SPSS统计软件对正交试验结果进行处理和分析。结果表明,3因素对硒多糖提取率影响大小为料液比>微波处理时间>水提时间,且红豆杉中硒多糖的最佳提取条件为料液比1∶80(W/V)、水浴浸提时间30 min、微波处理时间5 min。在此条件下,测得红豆杉中硒多糖含量为1.96%,硒多糖中硒含量为0.0555μg/g。

In this thesis, Taxus chinensis, a kind of precious medicinal plant that grows in Enshi where it is a high selenium areas in China, was used as experimental material. Solid-liquid ratio, water extraction time and microwave processing time were the three experimental factors, then orthogonal experiment was designed to find a more efficient way to extract and separate Se-polysaccharide from T. chinensis. Based on that, the phenol sulfuric acid method was used to detect the content of Se-polysaccharide in T. chinensis, and the atomic fluorescence method was used to detect the content of Se in Se-polysaccharide. In addition, statistical software, SPSS, was used to analyze and process the orthogonal experiment results. The results showed that the best conditions to extract this kind of polysaccharide were as follows： the solid-liquid ratio was 1∶80（W/V）, water extraction time was 130 min, microwave processing time was 5 min. Among these three factors, the influence order on polysaccharide extraction rate was solid-liquid ratiomicrowave processing timewater extraction time. Under these conditions, the content of Se-polysaccharide was up to 1.96%, selenium content in Se-polysaccharide was 0.0555 μg/g.