摘要
为了解兔肉质性状特征,给兔的品种选种选育提供理论依据,选择UCP1基因设计1对引物扩增其第3外显子及第2内含子部分序列,采用DNA池PCR反应,直接测序法快速检测比利时兔、新西兰兔和加利福尼亚兔3个兔品种UCP基因多态性。结果在新西兰兔中发现1个多态性位点(T387C),多态位点对UCP1基因的RNA结构产生影响,其最小自由能由-917.51 k J/mol变为-927.12 k J/mol,而其他2个品种没有检测到该突变。
In order to deeply understand the meat quality and provide basic information and scientific proof for better heredity selection of rabbit, the complete sequences of exon3 and part of intron2 region in UCP1 of rabbit were obtained by designing one pair of specific primers. DNA pools PCR and the direct sequencing were used to test the SNP site in Belgian rabbit, New Zealand rabbit and California rabbit. The results showed that only one SNP（G387C） site was found in New Zealand rabbit, and the polymorphism led to the changes of RNA secondary structure, its minimum free energy changed from-917.51 k J/mol to-927.12 k J/mol. However, there was no SNP site in the other two breeds.
出处
《广东农业科学》
CAS
CSCD
北大核心
2014年第24期138-141,共4页
Guangdong Agricultural Sciences
基金
贵州省科技支撑计划项目(黔科合NY字[2012]3013号)
