摘要
本文采用胶体几丁质为底物,研究了红曲霉液态发酵产几丁质酶.研究结果表明:胶体几丁质的添加能有效诱导红曲霉合成几丁质酶.在培养基中添加0.8%(W/V)胶体几丁质的同时,添加0.4%(W/V)葡萄糖,有利于红曲霉的生长以及几丁质酶的合成,发酵48h几丁质酶活力达到最高的0.3504U/mL.
The study aimed to explore the chitinase production by Monascus liquid ferment-ation.The chitinase enzyme assay was carried out with colloidal chitin as a substrate.The experimental results indi-cate that addition of colloidal chitin helps the accumulation of chitinase.The highest activity of chitinase of 0.350 4 U/mL was obtained at 48 hours in themedium with the glucose 0.4%(W/V)and colloidal chitin 0.8%(W/V).
出处
《安徽工程大学学报》
CAS
2014年第4期1-3,14,共4页
Journal of Anhui Polytechnic University
基金
国家大学生创新创业训练计划基金资助项目(201310363021)
芜湖市重点科技计划基金资助项目(芜科计字[2009]190号文)