霉变饲料中添加复方霉菌毒素吸附剂对肉鸡人工感染发生的影响

The effect of moldy feed supplemented with or without compound mycotoxin adsorbent on artificial infection in broiler chickens

本试验旨在研究霉变饲料和／或添加复方霉菌毒素吸附剂对肉鸡人工感染发生的影响。选取140羽1日龄健康的AA肉鸡随机分成7个组．每组4个重复。第1组为空白对照组，饲喂基础日粮；第2组饲喂霉变饲料；第3～7组以混合感染病鸡病变组织匀浆饮水制作人工感染疾病模型；第3～7组分别饲喂基础日粮、霉变饲料、霉变饲料＋复方霉菌毒素吸附剂、霉变饲料＋复方霉菌毒素吸附剂＋抗氧化剂、霉变饲料＋复方霉菌毒素吸附剂＋抗氧化剂＋中药增免剂。试验期35d。结果发现，肉鸡摄入霉变饲料与摄入正常饲料相比，提高肉鸡料重比和死淘率，显著降低肉鸡增重、胸腺指数、法氏囊指数，显著降低血清IBDV、H9N1v和NDV抗体水平（P〈0．05）。给人工感染肉鸡饲喂霉变饲料与饲喂正常饲料相比．显著提高料重比和死淘率，显著降低肉鸡增重、胸腺指数和脾脏指数，显著降低血清IFN-1、IL-2、IL-4和IL-12含量以及LTR，显著降低血清IBDV、H9N1V和NDV抗体水平（P〈0．05）。霉变饲料中添加复方霉菌毒素吸附剂能显著提高混合感染肉鸡增重，并降低料重比和死淘率（P〈0．05），显著提高胸腺指数、法氏囊指数（P〈0．05），显著提高血清IBDV、H9N1V和NDV抗体水平（P〈0．05）。可见，霉变饲料可导致肉鸡免疫功能抑制，加重肉鸡混合感染病情；复方霉菌毒素吸附剂能有效缓解霉变饲料对人工感染肉鸡生产性能、免疫功能和病情的不利影响，抗氧化剂和免疫功能增强剂可减轻霉变饲料的毒性作用。

This experiment was conducted to determine the effect of moldy feed and moldy feed supplemented with compound mycotoxin adsorbent on happening of mixed infection in broiler chickens. 140 one-day-old broiler chickens were randomly divided into 7 treatments with 4 replicates of 5 broiler chickens. Treatments were：l）negative control that was fed with basal diet,2）broiler chickens fed with moldy feed, 3） broiler chickens fed with basal diet,4）broiler chickens fed with moldy feed,5）broiler chickens fed with moldy feed supplemented with compound mycotoxin adsorbent,6）broiler chickens fed with moldy feed supplemented with compound mycotoxin adsorbent and antioxidant,7）broiler chickens fed with moldy feed supplemented with compound myeotoxin adsorbent, antioxidant and immunopotentiator. The broiler chickens in treatments 3 to 7 drinked aqueous solution of pathological tissue homogenate of the sick broilers to build mixed infection model. The trial lasted for 5 weeks. The results showed that, compared with normal feed, moldy feed intake of broiler chickens significantly increased death and culling rate, and decreased average daily weight gain, feed conversion ratio, the index of thymus and bursa of fabricius, antibody level of IBDV, H9N1V and NDV of serum in broiler chickens （P〈0.05）o Compared with normal feed, moldy feed intake of sick broiler chickens significantly increased death and culling rate, and decreased average daily weight gain, feed conversion ratio, the index of thymus and spleen, the content of IFN-γ IL-2, IL-4 and IL-12 of serum, LTR, the antibody level of IBDV,I-IgNN and NDV of serum in broiler chickens （P〈0.05）o Fed with moldy feed supplemented with compound mycotoxin adsorbent, the average daily weight gain, feed conversion ratio the index of thymus and bursa of fabricius the antibody level of IBDV,HgNIV and NDV of serum in artificial infected broiler chickens were increased （P 〈0.05）. Therefore,the moldy feed intake brought about immunosuppression in broiler chickens and aggravate the disease of artificial infected broiler chickens. Fed with moldy feed supplemented with compound mycotoxin adsorbent, the adverse effect of moldy feed intake on production performance, Immune function and disease of artificial infected broiler chickens is mitigated.