NF-κB核苷酸圈套技术制备imDC联合超声介导对抗同种异基因大鼠胰十二指肠移植排斥反应研究

Experimental Study of NF- κB Nucleotide Trap Technology to Prepare Im DC and Ultrasound Mediated Method Anti Pancreaticoduodenal Allogeneic Rat of Allograft Rejection

目的探讨NF-κB核苷酸圈套技术制备im DC联合超声介导抗同种异基因大鼠胰十二指肠移植排斥反应的作用。方法建立同种异基因大鼠胰十二指肠移植模型,分为对照组、NF-κB核苷酸圈套技术组、超声介导处理组及联合治疗组。检测细胞因子INF-γ、IL-4及IL-10水平及INF-γ、IL-4及IL-10 mRNA的表达;统计各组大鼠的生存率。结果联合治疗组生存周期最长,与其他组比较差异有统计学意义(P<0.05)。联合治疗组IL-4及IL-10水平最高,与其他组比较差异有统计学意义(P<0.05)。结论 NF-κB核苷酸圈套技术制备im DC联合超声介导可协同增加抗同种异基因大鼠胰十二指肠移植排斥反应。

Objective To investigate the NF- κB nucleotide trap preparation technology of im DC anti allogeneic pancreaticoduodenal allograft rejection in rats. Methods Establishing allogeneic pancreaticoduodenal transplantation model in rats,and dividing them into the control group,NF- κB nucleotide trap technology group,ultrasound mediated group and the combined group. The expression of cytokines INF-,IL- 4 and IL- 10 levels and INF- γ,IL- 4 and IL- 10 mRNA were determined. And the survival rate were also studied. Results The survival rate of the combined group was the longest and there was statistically significant difference compared with other groups（ P〈 0. 05）. The IL- 4 and IL- 10 level of the combined group was the highest and there was statistically significant difference compared with other groups（ P 〈0. 05）. Conclusion NF- κB nucleotide trap technology for preparing im DC combined with ultrasound can synergistically increase anti allogeneic pancreaticoduodenal allograft rejection in rats.