恒温箱加温对库存红细胞悬液携氧能力的影响

Effect of heating in a calorstat to the oxygen-carrying function of red blood cell

目的研究库存红细胞悬液在室温加温和恒温箱加温下红细胞携氧功能的变化。方法从4℃贮血冰箱中随机取出红细胞悬液40份,分别置于室温中(N组)或37℃恒温培养箱(H组)加温30 min,分别取样测定电解质、血气分析、游离Hb、ATP含量、2,3-DPG含量、氧亲和力(P50)。结果两组血pH值、血Na+都未有因加温而发生明显变化,血K+含量增加,游离Hb升高,葡萄糖下降但差异无统计学意义。加温后红细胞ATP含量、2,3-DPG含量、P50增加(P<0.05);与N组比较,H组升高更明显(P<0.05)。结论库血加温至37℃能够增加红细胞携氧功能,有助于提高红细胞输注的有效性。

Objective To study the change of the oxygen-carried function of stored red blood cells,which was warmed in room temperature and calorstat.Methods Randomly removed from 4 ℃storage refrigerator,red blood cell suspension were put in room temperature 30 min （group N）and 37℃ calorstat 30 min （group H）,and samples taken to examine electrolyte,blood gas,free Hb,ATP content,2,3-DPG content,oxygen affinity（P50）.Results In two groups,blood pH value and blood Na＋ did not change obviously due to heating.The content of blood K＋ and free Hb increased,glucose fell,but there was no statistically significant difference.The content of ATP and 2,3-DPG in eryth-rocyte increased and P50 increased after heating （P 〈0.05）,which increased more significantly in group H compared with group N （P〈0.05）.Conclusion Heating stored blood up to 37 ℃ can in-crease the oxygen-carried function of red blood cells,which contribute to improve the effectiveness of transfusion.