期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

大麻素1型受体在大鼠子宫内膜异位病灶神经生长中的作用 被引量：2

Cannabinoid receptor 1 controls nerve growth in ectopic cyst in a rat endometriosis model
原文传递
导出
摘要 目的：探讨大麻素1型受体（ CB1R）在子宫内膜异位囊肿组织神经生长中的作用。方法利用免疫荧光和免疫印迹方法检测CB1R激动剂、阻断剂对18只大鼠子宫内膜异位囊肿组织中泛神经标志物基因蛋白产物（PGP）9．5表达水平的影响。结果免疫荧光结果显示PGP 9．5在异位囊肿组织的神经纤维上表达，主要分布在囊肿门处。免疫印迹结果显示随着异位囊肿的生长，PGP 9．5（2周：0．38±0．05；4周：0．63±0．03；8周：0．80±0．07，P＜0．01）和CB1R（2周：0．48±0．04；4周：0．68±0．01；8周：0．80±0．03，P＜0．01）的蛋白表达水平均逐渐增加。另外，与对照组相比（0．75±0．01），鞘内注射CB1R激动剂 ACPA使囊肿组织内 PGP 9．5的蛋白表达增加（0．81±0．01，P ＜0．05），而CB1R阻断剂AM251使囊肿组织内PGP 9．5的蛋白表达下降（0．67±0．03，P ＜0．01）。结论大麻素CB1 R促进子宫内膜异位组织的神经生长。 Objective To investigate whether cannabinoid receptor 1 （CB1R） is involved in nerve growth in endometriosis-associated ectopic cyst.Methods The effect of CB1R agonist and antagonist on the expression of pan-neuronal marker protein gene product （ PGP ） 9.5 in ectopic cyst was examined by immunofluorescence and Western blot in endometriosis model of 18 rats.Results Immunofluorescence revealed that PGP 9.5 was expressed in the nerve fibers and was mainly distributed in the cyst hilum. Western blot revealed that the protein density of either PGP 9.5 （2 week：0.38 ±0.05;4 week：0.63 ± 0.03;8 week：0.80 ±0.07, P〈0.01） or CB1R（2 week：0.48 ±0.04;4 week：0.68 ±0.01;8 week：0.80 ±0.03, P〈0.01） in the ectopic cyst increased with cyst size.In addition, compared to control group （0.75 ±0.01）, PGP 9.5 expression in the ectopic cyst was promoted by CB1R agonist ACPA （0.81 ± 0.01, P 〈0.05）, and inhibited by CB1R antagonist AM251 （0.67 ±0.03, P 〈0.01） .Conclusions CB1R was involved in the nerve growth of ectopic cyst associated with endometriosis.
作者 赵倩倩 梁茜子 韩洪秀
机构地区 上海交通大学医学院附属第三人民医院病理科
出处 《中华病理学杂志》 CAS CSCD 北大核心 2014年第12期827-830,共4页 Chinese Journal of Pathology
基金 国家自然科学基金（81272927） 上海市科学技术委员会自然科学基金（12ZR1417500） 上海市教育委员会创新项目（13YZ038） 上海市宝山区科委项目（12-E-4）
关键词 子宫内膜异位症 神经纤维 受体 大麻酚 CB1 Endometriosis Nerve Fibers Receptor,cannabinoid,CB1
分类号 R711.71 [医药卫生—妇产科学]
  • 相关文献

参考文献17

  • 1Stratton P,Berkley KJ.Chronic pelvic pain and endometriosis:translational evidence of the relationship and implications[J].Hum Reprod Update,2011,17(3):327-346.
  • 2Zhang G,Dmitrieva N,Liu Y,et al.Endometriosis as aneurovascular condition:estrous variations in innervation,vascularization,and growth factor content of ectopic endometrialcysts in the rat[J].Am J Physiol Regul Integr Comp Physiol,2008,294(1):R162-H171.
  • 3Nagabukuro H,Berkley KJ.Influence of endometriosis onvisceromotor and cardiovascular responses induced by vaginaldistention in the rat[J].Pain,2007,132(Suppl 1):S96-S103.
  • 4Dmitrieva N,Nagabukuro H,Resuehr D,et al.Endocannabinoidinvolvement in endometriosis[J].Pain,2010,151(3):703-710.
  • 5Williams EJ,Walsh FS,Doherty P.The FGF receptor uses theendocannabinoid signaling system to couple to an axonal growthresponse[J].J Cell Biol,2003,160(4):481486.
  • 6He JC,Gomes I,Nguyen T,et al.The G alpha(o/i)-coupledcannabinoid receptor-mediated neurite outgrowth involves Rapregulation of Src and Stat3[J].J Biol Chem,2005,280(39):33426-33434.
  • 7Li L,Bender KJ,Drew PJ,et al.Endocannabinoid signaling isrequired for development and critical period plasticity of thewhisker map in somatosensory cortex[J].Neuron,2009,64(4):537-549.
  • 8Argaw A,Duff G,Zabouri N,et al.Concerted action of CBlcannabinoid receptor and deleted in colorectal cancer in axonguidance[J].J Neurosci,2011,31(4):1489-1499.
  • 9Marcondes FK,Bianchi FJ,Tanno AP.Determination of theestrous cycle phases of rats:some helpful considerations[J].Braz J Biol,2002,62(4A):609-614.
  • 10Vemon MW,Wilson EA.Studies on the surgical induction ofendometriosis in the rat[J].Fertil Steril,1985,44(5):684-694.

同被引文献15

  • 1谢幸.妇产科学[M].北京:人民卫生出版社,2013:274.
  • 2Nnoaham KE, Hummelshoj L, Webster P, et al. Impact of endome- triosis on quality of life and work productivity: a muhicenter study across ten countries[J]. Fertil Steril, 2011, 96:366-373.
  • 3Di SpiezioSardo A, Florio P, Fernandez LM, et al. The potential role of endometrial nerve fibers in the pathogenesis of pain during endo- metrial biopsy at office hysteroscopy[J]. Reprod Sci, 2015, 22:124- 131.
  • 4Zevallos HB, McKinnon B, TokushigeN, et al. Detection of the pan neuronal marker PGP9.5 by immuno-histochemistry and quantitatire PCR in eutopic endometrium from women with and without en- dometriosis[J]. Arch Gynecol Obstet, 2015, 291:85-91.
  • 5Morotti M, Vincent K, Brawn J, et al. Peripheral changes in endo- metriosisassociated pain[J]. Hum Reprod Update, 2014, 20:717- 736.
  • 6GrandPre T, Nakamura F, VartanianT, et al. Identification of the Nogo inhibitor of axon regeneration as a Reticulon protein[J]. Nature, 2000, 403:439-444.
  • 7Kim JE, LiS, GrandPre T, et al. Axon regeneration in young adult mice lacking Nogo-A/B[J]. Neuron, 2003, 38:187-199.
  • 8Simonen M, Pedersen V, Weinmann O, et al. Systemic deletion of the myelin-associated outgrowth inhibitor Nogo-A improves regen- erative and plastic responses after spinal cord injury[J]. Neuron, 2003, 38:201-211.
  • 9Tarjanne S, Ng CH, Manconi F, et al. Use of hormonal therapy is as- sociated with reduced nerve fiber density in deep infiltrating, recto- vaginal endometriosis[J]. Acta Obstet Gynecol Scand, 2015, 94:693-700.
  • 10Kim YA, Kim JY, Chang SH. Progesterone reduces neurofilament (NF)-positive nerve fibers in eutopic endometrium of patients with endometriosis and myomata[J]. Reprod Med, 2014, 59:481-487.

引证文献2

二级引证文献12

中华病理学杂志
2014年 第12期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部