CD55和CD59在慢性鼻-鼻窦炎中的表达及意义

Expression of CD55 and CD59 in chronic rhinosinusitis and its significance

目的 了解健康人及慢性鼻-鼻窦炎（chronic rhinosinusitis,CRS）患者鼻腔黏膜上皮细胞分泌的补体调节蛋白CD55及CD59的表达情况及其意义.方法 采用细胞培养及流式细胞仪等方法检测健康对照组（30例）及CRS患者（30例）鼻腔黏膜上皮细胞分泌的补体调节蛋白CD55及CD59的相对表达量.以SPSS 18.0软件进行统计学分析.结果 CD55在健康对照组鼻腔黏膜组织中的表达量为0.001 ±0.001,低于其在CRS组鼻腔黏膜组织中的表达量（0.067±0.028）,差异有统计学意义（t=-10.535,P＜0.01）.CD59在健康对照组鼻腔黏膜组织的表达量为0.879±0.005,高于其在CRS组中的表达量（0.238±0.034）,差异有统计学意义（t=83.416,P＜0.01）.结论 CRS患者鼻黏膜组织中CD55呈高表达而CD59呈低表达,这一机制可能参与了CRS的发病过程.

Objective To understand the expression of complement regulatory proteins CD55 and CD59,which secreted by epithelial cells of normal and chronic sinusitis patients.Methods Cell culture and flow cytometry were used to detect the expression of complement regulatory proteins CD55 and CD59.SPSS 18.0 software was used to analyze the data.Results CD55 was expressed both in normal nasal mucosa and mucosa of chronic sinusitis.The expression in normal group was 0.001 ±0.001,significantly lower than that in CRS group which was 0.067 ± 0.028 （t =-10.535,P ＜ 0.01）.CD59 was also expressed in the two groups.In normal group,the expression of CD59 was 0.879 ± 0.005,significantly higher than that in CRS group which was 0.238 ± 0.034 （t =83.416,P ＜0.01）.Conclusions The nasal mucosa in CRS patients showed low expression of CD55 and high expression of CD59.This mechanism may be involved in the occurrence of CRS.