间充质干细胞在小鼠体内可以向前列腺癌组织募集促进前列腺癌的生长

Mesenchymal stem cells could recruit to prostate cancer tissue and promote prostate cancer cell growth

目的探索间充质干细胞在小鼠体内与前列腺癌组织生长的关系,以期为前列腺癌的治疗探索新的靶点。方法野生型C57BL/6小鼠经致死剂量照射后,接受EGFP绿色荧光转基因C57BL/6小鼠的MSCs以及野生型小鼠全骨髓细胞的混合移植。1月后在骨髓移植成功的小鼠身上建立前列腺癌RM-1细胞皮下移植瘤模型,检测前列腺癌肿瘤的生长情况。同时以荧光显微镜显像技术及流式细胞仪技术观察、检测MSCs在小鼠前列腺癌组织中的表达情况。结果皮下接种RM-1前列腺癌细胞后,给予小鼠尾静脉注射MSCs的实验组肿瘤的生长速度要快于注射生理盐水的对照组,肿瘤重量分别为(3.730±0.632)g、(2.019±0.254)g(P<0.05);无论是骨髓移植后内源性的MSCs还是直接尾静脉注射的外源性MSCs都可以在前列腺癌组织中检测到。结论内源性以及外源性的MSCs都可以向前列腺癌组织趋化募集,并促进前列腺癌的生长。

Objective To explore the relationship between mesenchymal stem cells with prostate cancer growth in mice , in order to find new targets for the treatment of prostate cancer .Methods C57BL/6 wt‐mice received lethal irradiation ,ac‐cepted mixed EGFP transgenic C57BL/6 mouse MSCs and wild‐type mice bone marrow cells .Then subcutaneous tumor model of RM‐1 prostate cancer cell was established and the growth of prostate cancer was detected .EGFP‐MSCs cells in prostate cancer tissues were observed and detected with fluorescence microscopy imaging techniques and flow cytometry .Results Af‐ter RM‐1 prostate cancer cells were subcutaneous inoculated ,the tumor growth rate of the experimental group receiving intra‐venous injection of MSCs ,was higher than that of the control group which received NS .Tumor weights were （3 .730 ± 0 .632） g and （2 .019 ± 0 .254）g respectively ,（P〈 0 .05） .Either endogenous MSCs from bone marrow transplantation or exogenous MSCs from direct intravenous injection could be detected in prostate cancer tissues .Conclusion Endogenous and exogenous MSCs cells can be chemotacticly recruited to prostate cancer to promote cancer growth .