Par3、Par6及aPKC蛋白在胃癌发生、发展中的意义

Expression of Par3,Par6 and a PKC in gastric carcinoma and their significance

目的探讨Par3、Par6和a PKC在胃癌中的表达及意义。方法采用免疫组化法分别检测Par3、Par6和a PKC三种蛋白在153例胃癌各个位点(即胃癌黏膜层、肿瘤中心、侵袭前沿)及淋巴结转移灶中的表达,并以正常胃黏膜作为对照。结果Par3、Par6和a PKC在胃癌组织各个位点的表达水平均显著低于正常胃黏膜(P<0.01);Par3、Par6、a PKC在胃癌中的表达下调率高于肠型和混合型胃癌(P<0.05,P<0.01,P<0.01),Par6和a PKC在侵袭至浆膜及浆膜外病例中的表达下调率显著高于侵及黏膜及肌层的病例(P均<0.01),伴有淋巴结转移病例中Par6的表达低于不伴有淋巴结转移病例(P>0.05)。结论Par3、Par6和a PKC表达下调促进胃癌的发生、发展。

Purpose To investigate the expression of Par3, Par6 and aPKC in gastric carcinoma and their significance. Method Ex-pression of Par3, Par6 and aPKC, by using immunohistochemistry, was detected in different sites of gastric carcinoma （ including the gastric carcinoma in gastric mucosa, the central area and the invasive front of gastric carcinoma） and the lymph node metastasis, using normal gastric mucosa as controls. Results Expression of Par3, Par6 and aPKC in different sites of gastric carcinoma was lower than in that of normal gastric mucosa （P〈0. 01）. Expression of Par3, Par6 and aPKC was obviously lower in gastric carcinoma with gastric phenotype than in that with intestinal phenotype and mixed phenotype of gastric carcinoma （P〈0. 05, P〈0. 01, P〈0. 01）;the rate of down-regulation of Par6 and aPKC in gastric carcinoma with invasion to ectoptygma and out of ectoptygma was obviously higher than that in gastric carcinoma which located at mucosa and under mucosa （P both〈0. 01）, and the rate of down-regulation of Par6 in gas-tric carcinoma with lymph node metastasis was obviously lower than that with no lymph node metastasis （ P〉0. 05 ） . Conclusions The down-regulation expression of Par3, Par6 and aPKC may promote the carcinogenesis and progression of gastric carcinoma.