非小细胞肺癌中MMP-9和MMP-13的表达及意义

Expression of MMP-9 and MMP-13 in non-small cell lung cancer and their significance

目的分析MMP-9和MMP-13在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达及相关性,探讨二者与NSCLC临床病理特征及预后的关系。方法采用免疫组化SP法检测88例NSCLC和18例癌旁正常肺组织中MMP-9、MMP-13的表达。结果 MMP-9与MMP-13在NSCLC中的阳性率均高于癌旁正常肺组织(P<0.05)。MMP-9表达与患者年龄、分化程度、淋巴结转移有关(P<0.05);MMP-13表达与分化程度、淋巴结转移有关(P<0.05)。NSCLC中MMP-9与MMP-13的表达呈正相关(P<0.05)。MMP-9和MMP-13阳性组的生存率均低于阴性组,差异有显著性(P<0.05);且肿瘤大小、淋巴结转移和MMP-13阳性表达与NSCLC的预后密切相关(P<0.05)。结论 MMP-9与MMP-13均与NSCLC的侵袭、转移及预后密切相关,MMP-13可能主要通过激活MMP-9途径来参与NSCLC的侵袭、转移。

Purpose To explore the role of expression of MMP-9 and MMP-13 in non-small cell lung cancer （ NSCLC） , and to investi-gate their association with the clinicopathologic feactures and prognosis of NSCLC. Methods The expression of MMP-9 and MMP-13 was detected in 88 NSCLC tissues and 18 adjacent normal tissues by immunohistochemistry SP method. Results The expression of MMP-9 and MMP-13 was higher in NSCLC than that in adjacent normal tissues （P〈0. 05）, which was positively correlated with dif-ferentiation and lymph node metastasis （P〈0. 05）. The expression of MMP-9 was positively correlated with age （P〈0. 05）, which was positively correlated with the expression of MMP-13 in NSCLC （ P 〈0. 05 ） . The survival rates in positive expression group of MMP-9 and MMP-13 were significantly lower than that of the negative expression group by log-rank method comparing survival curves （P〈0. 05）. Cox model analysis showed that the tumor size, lymph node metastasis and MMP-13 positive expression were closely relat-ed with the prognosis of NSCLC （P〈0. 05）. Conclusion MMP-9 and MMP-13 both are associated with the metastasis, invasion and prognosis of NSCLC, and MMP-13 may mainly activate MMP-9 to participate the invasion and metastasis of NSCLC.