纳米银改性钛片对口腔常见致病菌抗菌性能实验研究

Anti-bacterial testsof silver nanoparticle-modified titanium surface

目的:通过纳米银改性钛片对牙龈卟啉单胞菌和伴放线放线杆菌抗菌能力的观察,探讨口腔种植体颈部钛表面抗菌改性的可行性。方法:实验组为纳米银改性钛片,对照组为光滑钛片,每组各10片。通过贴膜法和抗粘附实验两种方法检测纳米银改性钛片的抗菌性能。结果:贴膜法显示培养24 h后纳米银改性钛片对牙龈卟啉单胞菌和伴放线放线杆菌的抗菌率达到90%;抗粘附实验显示牙龈卟啉单胞菌散在分布,且对照组多于实验组,伴放线放线杆菌散在分布对照组钛片上大量粘附,呈片状或团状集聚在一起。结论:纳米银改性后的钛片抗菌性能突出,是一种具有前景的抗菌生物植入材料。

Objective：Porphyromonas gingivalis（Pg） and actinobacillusactinomycetemcomitans（Aa） were obtained to test the anti-bacterial effect of the Ti-nAg surface and to investigate the possibility of surface modification of titanium which used in dental implant. Method：nano-silver modified titanium plate as test group,while smooth titanium plate as control group,each group has 10 plates. the SEM exanimation of anti-adhesive efficacy testwas utilized. Result： Two type bacteria, Porphyromonas gingivalis（Pg） and actinobacillusactinomycetemcomitans（Aa）were obtained to test the anti-bacterial effect of the Ti-nAg surface. After 24-hour incubation,over 90 % of bacteria have been killed on the Ti-nAg surface,and the SEM exanimation of anti-adhesive efficacy test showed that there were less bacteria attached to Ti-nAg surface than to control Titanium surface,the inhibition of the bacterial growth depends on a sufficient concentration of silver ions in thesurrounding aqueous environment. Conclusion：These data suggest that silver nanoparticle-modified titanium is a promising material with an antibacterial property that may be used as an implantable biomaterial.it is possible that the silver dissociated from the titanium surface did not reach a concentration that is sufficient to inhibit bacterial growth.