摘要
目的 探讨大鼠阴茎海绵体神经（CN）侧支对阴茎勃起的作用及对建立CN损伤动物模型的影响.方法 36只3～5月龄雄性SD大鼠,其中6只在外科显微镜下详细解剖盆腔神经节及其分支;另30只大鼠随机分成三组：Ⅰ假手术组,Ⅱ双侧CN主支切断组和Ⅲ双侧CN主支和侧支均切断组.分别于术后7d和30 d皮下注射阿朴吗啡,观察0.5 h内阴茎勃起情况.然后,在大鼠阴茎海绵体内注射神经示踪剂荧光金,5d后取大鼠盆腔神经节,冰冻切片后荧光显微镜下观察.结果 在前列腺背侧叶表面,盆腔神经节向尿道膜部方向发出CN主支和数根极为细小的分支.术后7d,Ⅰ组勃起次数为2.0±0.7,而Ⅱ、Ⅲ组大鼠无勃起反应;术后30d,Ⅲ组仍无勃起反应,Ⅱ组大鼠勃起次数0.9±0.7,但仍低于Ⅰ组（2.8±0.6）（P＜0.05）.荧光显微镜下计数,Ⅱ组盆腔神经节中可见较多荧光金阳性着色细胞,而Ⅲ组只可见极少数荧光金阳性着色细胞（P＜0.01）.结论 大鼠盆腔神经节发出的细小分支中含有对阴茎勃起有部分支配作用的CN侧支,建立大鼠CN损伤阴茎勃起功能障碍（ED）动物模型时应考虑CN侧支的影响.
Objective To explore the role of collateral branch of cavernous nerves （CN） in penile erection in rats and its importance in establishing CN injury rat models.Methods Thirty-six 3～5 months old male SD rats were used.Six rats were used to identify and observe the branches of major pelvic ganglion （MPG） under the operating microscope,the other 30 rats were divided into 3 groups randomly,including （Ⅰ） sham-operated group,（Ⅱ） bilateral main branch of CN ablated group and （Ⅲ）bilateral main and collateral branches of CN ablated group.The erectile ability of rats were evaluated with apomorphine test respectively 7 days and 30 days after surgery.Then 4％ flluoro-gold solution were injected into bilateral corpus cavernosum of penis in all rats under anesthesia,and the rats were sacrificed and their bilateral MPGs were obtained for tracing the flluoro-gold under the fluorescent microscope 5 days later.Results The main branch of CN and some fine fibers arised from the MPGs were identified on the surface of dorsolateral lobes of the prostate.7 days after surgery,the times of rat penile erection in group was 2.0 ± 0.7,but no penile erection was found in both group Ⅱ and Ⅲ.30 days after surgery,the group Ⅱ rats still showed no erection,the erectile response was appeared in group Ⅱ rats after administration of apomorphine,but the erection times of group Ⅱ （0.9 ± 0.7） was still less than that of group Ⅰ （2.8 ± 0.6）（P＜0.05）.5 days after injection of fluoro-gold solution,much more brighter positive stained neural cells were observed in MPGs of group Ⅱ than those of group Ⅲ （P＜0.01）.Conclusions Some tiny nerve fibers,the collateral branches of CN derived from MPG,may play a part role in penile erection of rats,and the influence of these collateral branches should be taken into consideration when creating a erectile dysfunction rat model induced by CN injury.
出处
《实验动物与比较医学》
CAS
2014年第6期433-437,共5页
Laboratory Animal and Comparative Medicine
基金
国家自然科学基金资助项目（81070483）
