六种常用实验动物比较组织学共享数据库的初步建立

Preliminary Establishment of Shared Database about Comparative Histology in 6 Species of Laboratory Animals

目的 建立常用实验动物比较组织学数据库.方法 选取小鼠、大鼠、豚鼠、兔、犬和食蟹猴6种实验动物的所有主要器官和组织,制作石蜡切片后经HE染色,在镜下观察各种组织的形态特征并拍照描述.对采集好的图片、文字信息采用SQL Server 2008数据库管理软件,汇编开发成易于查询的比较组织学数据库系统.结果 通过6种实验动物的所有组织和器官的形态进行比较分析,获得了这些组织器官形态学差异的特征,镜下拍取具有组织特征的显微图片,对每张图片进行文字描述,建立了常用实验动物比较组织学数据库,对其内容进行初步分析,实现了界面友好、便于查询和资源共享的目的.结论 建立了常用实验动物主要器官和组织的比较组织学数据库,可为生命科学领域中动物实验的病理学研究及疾病动物模型研究提供使用方便的组织形态学参考资料.

Objective To establish a comparative histology database of commonly used laboratory animals.Methods All the main organs and tissues from 6 species of laboratory animals,the mouse,rat,rabbit,guinea pig,dog and cynomolgus monkey,were collected.After embedded by paraffin,sectioned and stained with hematoxylin and eosin,tissue sections were observed under microscope and described.The comparative pathology database were established by SQL Server 2008 database management system.Results Tissues were compared among these 6 species of laboratory animals and distinctive pictures were got plused with descriptions.A user-friendly,inquiry conveniently and shared comparative histology database were established.Conclusion The establishment of comparative histology database are benefit for research of animal pathology and disease model in the field of bio-medicine.