基于高效液相色谱-二极管阵列检测法测定白花蛇舌草中5个环烯醚萜成分的含量

Contents of five iridoids in Ooldenlandia diffusa( Wind. ) Roxb. based on HPLC-DAD

目的：建立高效液相色谱-二极管阵列检测法同时测定白花蛇舌草中5个环烯醚萜成分含量的方法,为制定白花蛇舌草质量标准提供参考。方法：采用ZORBAX Extend-C18（250 mm×4.6 mm,5μm）色谱柱;以乙腈（B）-0.05%磷酸溶液（A）梯度洗脱,检测波长238 nm,流速1.0 ml·min^-1,柱温30℃。结果：鸡屎藤次苷甲酯、车叶草酸、车叶草苷、反式6-O-香豆酰鸡屎藤次苷甲酯和反式6-O-阿魏酰鸡屎藤次苷甲酯各自浓度范围内线性关系良好（r〉0.999 6）,平均加样回收率为97.20%-100.6%,RSD均小于3%（n=6）。应用所建方法测定了不同批次白花蛇舌草中5个环烯醚萜成分的含量。结论：该方法简单、快速、准确,可为白花蛇舌草药材质量控制提供参考。

OBJECFIVE To develop a method of high performance liquid chromatography coupled with a photodiode array detector for the content determination of scandosidemethyl ester, asperulosidic acid, asperuloside, （E） -6-O-p-coumaroyl scandoside methyl ester and （E） -6-O-feruloyl scandoside methyl ester in Oldenlandia diffusa （Wind.） Roxb. METHODS The analyses were performed on a ZORBAX Extend-C18 column （250 mm × 4. 6 mm, 5 μm） eluted with aeetonitrile （B）-0. 05% phosphoric acid （A） in gradient mode at a flow rate of 1.0 ml·min^-1, a detection wavelength of 238 nm, and a column temperature at 30℃. RESULTS Five iridoids have good linear relationship in their respective concentration range （r〉0. 999 6）, the mean recovery rates were 97. 20% - 100. 6%, RSD 〈3 % （n = 6）. Five iridoids of different batches of Oldenlandia diffusa （Wind.）Roxb. were determined by using our HPLC-DAD. CONCLUSION The method is simple, rapid and accurate, and can be used as the quality control for Oldenlandia diffusa （Wind.） Roxb..