摘要
通过观察WNT3在不同分化程度胃癌中的表达差异及其与胃癌患者临床病理资料之间的关系,来探讨WNT3在胃癌发病过程中的作用.本研究采用Real time PCR及免疫组化的方法,对比分析了46例高分化、55例中分化、58例低分化胃癌及15例正常组织中WNT3基因在转录和翻译水平的表达情况.Real time PCR结果显示,在不同分化程度的胃癌组织中,WNT3mRNA的表达较正常对照组织显著升高,差异具有统计学意义(P<0.05).免疫组化结果显示,与正常对照组相比,高分化、中分化、低分化胃癌组织中wnt3蛋白的含量逐渐增高,不同分化程度的胃癌组织与正常组间的差异具有统计学意义(P<0.05).临床资料分析表明,wnt3在胃癌中的表达与患者的性别、年龄等无关,而与淋巴结转移有关.因此,wnt3可能是启动WNT经典信号,参与胃癌发生、发展的重要分子.
The WNT3 expression in gastric carcinoma with different differentiation degrees, which from different patients,was examined to explore the roles of WNT3 in the pathogenesis of human gastric carcinoma. WNT3 transcription and translation levels were compared among 46 well, 55 moderately and 58 poorly differentiated gastric carcinoma tissues by real-time PCR and immuno- histochemistry methods. Normal gastric tissue was used as control. As revealed by real-time PCR results,WNT3 mRNA expression in different differentiated gastric carcinoma were significantly higher than control group （P〈0.05）. The expression levels of wnt3 protein in different differentiated gastric carcinoma were significantly higher than in control group （P〈0.05）,and the protein levels gradually increased in well, moderately and poorly differentiated tissues, respectively. Moreover,the wnt3 protein level was only affected by whether the gastric carcinoma had metastasized to the lymph node, but not the sex and age of patients. Therefore, wnt3 could be an important factor to start the WNT classic signal pathway,then take part in the gastric carcinoma genesis and development process.
出处
《内蒙古大学学报（自然科学版）》
CAS
北大核心
2015年第1期66-70,共5页
Journal of Inner Mongolia University：Natural Science Edition
基金
内蒙古自治区教育厅重点项目(NJZZ12149)
内蒙古自治区卫生厅项目(2010218)
内蒙古教育厅自然科学项目(NJZY11121)
