大孔吸附树脂分离蝉蜕抗凝纤溶组分的研究

Study on the separation and purification of anticoagulant and fibrinolytic components from periostracum cicada with macroporous resin

目的:研究大孔吸附树脂分离蝉蜕水提醇沉物中抗凝纤溶组分的工艺条件。方法:以比吸附量及解吸率为主要参考指标,辅以凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)及血凝-纤溶药效指标,采用NKA-9型大孔吸附树脂对蝉蜕水提醇沉物进行动态吸附分离,确定大孔吸附树脂分离纯化蝉蜕水提醇沉物中抗凝纤溶组分的最佳工艺条件。结果:选取NKA-9型大孔吸附树脂,径高比为1:12,以浓度10mg/m L的25m L样液,上样,2BV/h的流速连续通过树脂柱。吸附完全后,用6BV 50%乙醇进行洗脱。结论:该工艺稳定可行,可有效地用于蝉蜕水提醇沉物中抗凝纤溶组分的分离纯化。

Objective： To study the process conditions of separation and purification of anticoagulant and fibrinolytic components from periostracum cicada with macroporous resin. Methods： The anticoagulant and fibrinolytic components from alcohol-precipitation extracts of periostracum cicada were dynamically separated and adsorbed by the NKA-9 macroporous resin. The main indexes that adsorption capacity and resorption rate and the secondary indexes that the prothrombin time （PT）, the activated partial thromboplastin time （APTT） and the blood clots-fibrinolytic dynamic figure were used to determine the optimal process conditions. Results： The optimal purification conditions were as followed： selecting the NKA-9 macroporous resin according to the diameter and height ratio of 1：12, loading sample concentration at 10mg/mL and volume at 25mL, passing resin according to flow rate at 2BV/h and eluting with 6BV of 50% ethanol in last step. Conclusion： The process condition was stable and feasible, which could be effectively used to separate and purify anticoagulant and fibrinolytic components in the periostracum cicada.