祛风药对大鼠肝纤维化肝组织病理损伤的影响

Influence of expelling wind herbs on pathologic damage in rats with liver fibrosis

目的:观察祛风药对大鼠肝纤维病理损伤的影响。方法:将SD雄性大鼠80只随机分为正常对照组(8只)与模型组(72只)。模型组用50%CCl4橄榄油溶液皮下注射制造大鼠肝纤维化模型共12周,8周造模成功后,将模型大鼠随机分为祛风药组(防风、羌活、白芷)、下瘀血汤组、秋水仙碱组、病理模型组,继续造模的同时分别给予相关药物干预,观察各组大鼠一般情况变化和肝组织的病理组织学变化。结果:与病理模型组比较,光镜下秋水仙碱组大鼠肝细胞可见假小叶,下瘀血汤组和祛风药组大鼠光镜下可见灶性正常肝细胞索,未见肝假小叶形成,且祛风药组优于下瘀血汤组。电镜下可见秋水仙碱组、祛风药组和下瘀血汤组都能抑制细胞器变性,祛风药组的抑制效果优于秋水仙碱组和下瘀血汤组。结论:祛风药对大鼠肝纤维化有一定的抑制作用,可为临床抗肝纤维化提供新的治疗思路。

Objective： To observe the influence of expelling wind herbs on pathologic damage in rats with liver fibrosis. Methods： Eighty SD male rats were randomly divided into normal control group （n=8） and model group （n=72）. The rat model of hepatic fibrosis in model group was made by subcutaneous injection 50% CC14 olive oil solution in 12 weeks. After 8 weeks successful modeling, the model rats were randomly divided into expelling wind herbs group （Fangfeng, Qianghuo, Baizhi）, Xiayuxue Decoction group, colchicine group, pathological model group. Rats were continuing to model while intervening through given related drugs respectively. General and histopathological changes of liver tissue were observed in each group. Results： Compared with pathological model group, the pseudolobule in rat liver cells in colchicine group were observed by using a light microscope. Normal liver cell cord in Xiayuxue Decoction group and expelling wind herbs group was observed by using a light microscope, no formation of liver pseudolobules, and the effect of expelling wind herbs group was better than in Xiayuxue Decoction group. All treatment groups could inhibit cell organelles degeneration under an electron microscope. The inhibitory effect of expelling wind herbs was superior to colchicine group and Xiayuxue Decoction group. Conclusion： Expelling wind herbs had some inhibition on hepatic fibrosis in rats, which would provide new treatment ideas in clinical.