摘要
建立了兽药制剂中利巴韦林的高效液相色谱测定方法,对兽药制剂中利巴韦林的提取、净化和色谱条件进行了优化。以p H 2.5的水作为溶剂进行超声提取,提取液经C18固相吸附净化,采用高效液相色谱法进行定性和定量分析。在优化条件下,利巴韦林的线性范围为0.1-50.00μg/m L,相关系数R2为0.999 3,检出限为0.017μg/m L。加标水平为10、50、100μg/g时,平均加标回收率73.7%-116.8%。该方法快速、准确、灵敏度高、重复性好,能满足中兽药制剂中利巴韦林含量的检测要求。用该方法对全国12个省份的158种市售兽药样品进行检测,利巴韦林违禁添加总检出为4.3%。
A determination method of Ribavirin in veterinary drugs used high performance liquid chromatography was established.The procedures of extraction,purification and chromatography condition were optimized. Veterinary drugs were extracted with water（ p H 2. 5） and purified by C18 solid phase adsorption packing and then qualitative and quantitative assayed by HPLC. Under the optimized conditions,the calibration curves of Ribavirin were linear in the range of 0. 1 - 50. 0 μg / m L with correlation coefficients（ R2） of 0. 999 3 and detection limits of 0. 017 μg/m L. The average spiked recoveries at the spiked levels of 10,50,100 μg/g were in the range of 73. 7% - 116. 8%. The results showed that the method was rapid,accurate,sensitive and reliable,and could satisfy the requirements for analysis on Ribavirin in veterinary drugs. Illegally added Ribavirin in 158 veterinary drugs collected from 12 provinces were detected by this method,the total detectable rate of the Ribavirin was 4. 3%.
出处
《化学试剂》
CAS
北大核心
2015年第1期49-52,共4页
Chemical Reagents
基金
2014年国家畜禽产品质量安全风险评估基金资助项目(GJFP2014007)
