川芎嗪对d-半乳糖所致衰老大鼠学习记忆功能和海马CA1区长时程增强的影响

Effects of Tetramethylpyrazine on Learning and Memory Capability and Long-term Potenti ation in Hippocampal CA1 of the D-galactose Induced Aging Rats

目的:观察川芎嗪对d-半乳糖所致AD模型大鼠学习记忆功能和海马Schaffer侧枝-CA1通路LTP的影响,并探讨其可能机制。方法:24只大鼠随机分为对照组、模型组和TMP组,以d-半乳糖注射建立衰老大鼠模型,TMP组腹腔注射TMP干预。水迷宫实验检测大鼠学习记忆能力,电生理实验比较强直刺激前后海马CA1区PS幅度变化。结果:在水迷宫实验中,模型组大鼠潜伏期延长,错误次数增多,TMP明显改善衰老大鼠学习记忆功能。电生理实验中,模型组强直刺激后平均PS增幅明显低于对照组,TMP组能提高衰老大鼠PS增幅。结论:TMP能提高衰老大鼠的空间学习和记忆能力,其机制可能与TMP能改善d-半乳糖对大鼠海马CA1区LTP的抑制有关。

Objective To observe the effects of tetramethylpyrazine on learning and memory capability and synaptic plasticity in hippocampal CA1 of aging rats induced by D -galactose.Methods 24 SD rats were randomized into control group,The models of aging rats were made by given injecting D -galactose （120mg·kg^ -1 ·d^ -1 ）,The rats in the TMP group were treated with TMP solution （10mg·kg^ -1·d ^-1 ,ip ）,the rats in the control group were treated with the same dose of saline.After 28 days,the learning and memory capability were detected in Morris water maze.At the end of the experiment,electrophysiological method was used to observe the LTP in hippocampal CA1 area.Results The Morris water maze test showed that TMP significantly improved learning and memory performance in aging rats.In the electrophysiological experiments,PS amplitude significantly decreased in model group versus control, and TMP treatment restored PS amplitude.Conclusion TMP can improve learning and memory capability and synaptic plasticity in the hippocampal CA1 of aging rats,and this effect could be attributed to improvement of synaptic dysfunction.