胃癌组织中胸苷磷酸化酶表达和微血管密度的研究

Expression of TP and study of microvessel density in gastric carcinoma

目的探讨胸苷磷酸化酶(TP)在胃癌组织中的表达、微血管密度(MVD)与胃癌临床病理特征及预后间的关系。方法采用免疫组化SP法检测77例胃癌组织及25例癌旁正常胃黏膜组织中TP的表达,选择CD34作为血管内皮细胞标记物,对CD34阳性血管进行MVD计数。结果 TP和MVD在胃癌组织中的表达率分别为59.74%和(34.99±9.46)%,明显高于在癌旁正常组织中的表达12.0%和(12.65±4.00)%,差异有统计学意义(P<0.05)。TP和MVD在胃癌组织中的表达与淋巴结转移、浸润深度及临床分期有关(P<0.05),而与肿瘤大小、分化程度及肿瘤大体分型无关。TP阴性表达者的5年总生存期明显高于阳性表达者,差异有统计学意义(P<0.05)。MVD≤33组的胃癌患者5年总生存期明显优于MVD>33组,差异有统计学意义(P<0.05);经相关分析,TP阳性表达率与MVD呈正相关(P<0.05)。结论 TP在胃癌组织中存在高表达,且其表达和MVD呈正相关,并与胃癌的生长、浸润、转移及预后有关。

Objective To detect the expression of TP and correlation of microvessel density （ MVD） in gastric carcinoma and to discuss the relationship between the clinical and pathological characteristics and prognosis of gastric cancer. Methods The expressions of TP,MVD were studied by immunohistochemistry in 77 gastric carcinoma specimens and 25 normal tissues. CD34 was chosen as the vascular endothelial cell markers to count MVD. Results The rate of positive expression of TP and MVD in gastric cancer tissue was 59. 74% and 34. 99 ± 9. 46,respectively,which were significantly higher than those in neighboring gastric cancer tissue that were 12. 0%and 12. 65 ± 4. 00,respectively. All of the differences had statistically significant （all,P0. 05）. The five-year overall survival was higher in patients with tumors negative for TP than in those with tumors positive for TP;the five-year overall survival was lower in patients with tumors having high MVD score than in patients with tumors having low MVD score （P〈0. 01）. In gastric carcinoma,the expression had a positive correlation with TP and MVD （P〈0. 01）. Conclusion TP and MVD are related to the growth,infiltration, metastasis and prognosis of gastric carcinoma.