食品及其包装材料中双酚A的测定方法研究

Determination of Bisphenol A in Food and Plastic Food Containers

目的基于阳离子聚合物（PDDA）能诱导金纳米粒子聚集,PDDA与单链核酸之间的电荷吸引效应能够阻止PDDA的诱导作用,以及BPA核酸适配体的特异性,构建一种新型生物传感器检测分析双酚A含量的方法。方法在含有特定浓度的单链DNA金纳米粒子溶液中依次加入双酚A和阳离子聚合物,室温下混匀反应,最后利用紫外检测器扫描整个体系光谱图。研究特定波长下的吸光值随双酚A浓度的变化值。结果在最佳优化条件下,检测体系在特定波长处吸光值随BPA浓度的不同而发生变化,在质量浓度为1~50 ng/m L时呈良好线性关系,检出限为0.6 ng/m L。将该检测体系应用于BPA类似物的检测,有较为明显的专一性。应用该法测定湖泊水中BPA标样的含量,回收率为95.6%~108.0%。结论该方法对工业生产和食品安全中BPA的痕量检测具有一定的应用前景。

To design a new kind of biosensor for detection of bisphenol A（BPA） based on the fact that the aggregation of gold nanoparticles（Au NPs） could be induced by Phthalic Diglycol Diacrylate（PDDA）, and that oligonucleotides combine with PDDA to prevent Au NPs from aggregation, and the specificity of the anti-BPA aptamer. Au NPs solution containing anti-BPA aptamer and PDDA at a certain concentration was set as the reaction system. Upon the addition of BPA and reaction at room temperature, the specific wavelength of light absorption value changed along with the concentration of BPA which could be determined by UV-Vis spectrometry. Under the optimal conditions, a low limit of detection of 0.6 ng/m L was obtained in the range of 1~50 ng/m L. In addition, the specificity was outstanding among analogues of BPA. The recovery rate in lake water samples spiked with BPA could reach 95.6% to 108.0%. A convenient and sensitive method was successfully used to determine BPA, and the method has a certain application prospect for industrial production and food security in the trace detection of BPA.